Expressing Of Angiopoietin-2 And VEGF In Transitional Cell Carcinomar Of Bladder And Their Relation To Tumor Angiogenesis And Clinical Significance

OBJECTIVE To investigate the relationship between Angiopoietin-2(Ang-2),VEGFexpression and tumor Angiogenesis and clinicopathological variables,clinical outcomein patients with bladder cancer .In order to evaluate the roles of Ang-2,VEGFexpression in the tumor angiogenesis of bladder cancer, The relationship between theexpression of angiopoietin-2 and VEGF and Microvessel Density(MVD) werestudied.MATERIALS AND METHODS The expression of Ang-2 ,VEGF and MVD were measued byimmunohistochemistry in tissue sections from 80 transitional cell carcinomas of thebladder and 10 cases of normal bladder specimens.The differene of their expressionamongs three groups,the relationship between them and the pathological grades andclinical stages, outcome of bladder cancer, the relationship between the expression ofthemselves and tumor angiogenesis were analysed.RESULTS Ang-2 was noted in both bladder tumor cell and normal bladder tissue. VEGF wasnoted in bladder tumor cell. There were no statistically significant relationshipsbetween Ang-2,VEGF expression and the patient's age or gender. The intensity ofexpression of Ang-2,VEGF was increased with the increasing of grades and stages ofthe tumor(p<0.05); the expression of Ang-2,VEGF was found to be more intense inhigher grades(G2-G3),and invasive(≥T2)bladder cancer than that in lower grade (G1)and superficial bladder cancer (p<0.05); There were no statistically significantrelationships between MVD expression and the patient's age or gender. Theexpression of MVD was found to be increased with the increasing of grade of thetumor (p<0.05). The expression of MVD was found to be much more in highergrades(G2-G3),and invasive(≥T2)bladder cancer than that in lower grade(G1)andsuperficial bladder cancer (p<0.05);MVD in Ang-2 positive group is larger than innegative group. MVD in VEGF positive group is larger than in negative group. MVDin VEGF positive and Ang-2 positive is the largest than the other groups. The strongexpression of Ang-2 suggest poor prognosis. The group of VEGF positive and Ang-2strong expression is poorest prognosis.CONCLUSIONS These results suggest that Ang-2 VEGF and MVD overexpression wereassociated with tumor progression. Angiogenesis plays a very important role in themalignant progression of the tumor. The expressions of Ang-2 and VEGF had acooperation in the promotion of tumor angiogenesis of bladder cancer. The strongexpression of Ang-2 and VEGF suggest poor prognosis .