| BACKGROUND: Human colorectal carcinoma is one of the most common malignant tumous in china. The development of colorectal carcinoma preceeds through a series of genetic changes involving the activation of oncogenes and loss of tumor suppressor genes. Tob is a member of Tob/BTG anti-proliferative protein family that includes BTG2,BTG1,Tob, Tob2, and ANA in humans. Exogenous overexpression of Tob protein suppressed the growth of NIH/3T3 cells and induced G0/G1 arrest. Recent evidence suggests that Tob could be a tumor suppressor gene in lung cancer. However, the expression of Tob and the role of Tob in human colorectal cancer are not very clear. In the light of this information, we conducted this study to investigate the expression of Tob and the role of Tob in human colorectal cancer .AIM:To study the expression leves of Tob gene in human colorectal cancer tissues, and their corresponding para-cancerous tissues , and to investigate whether this gene plays a role in the pathogenesis of colorectal cancer. The correlation of expression of the Tob gene with clinicopathological characteristics of colorectal cancer was also analyzed.METHODS: Quantitative real time RT-PCR was used to detect the expression of Tob mRNA in 31 colorectal cancers. RESULTS: Compared with normal tissues, upregulation of Tob mRNA was observed in 31colorectal cancer tissues (t= 4.09, p= 0.000) . The expression value of TobmRNA in Dukes A,B,C,D was 3.250+1.484, 2.467 + 1.246, 1.167+0.408and 1.000+0.010, respectively. Analyzed by one-way ANOVA,there weresignificant differences in the expression of Tob in defferent Dukes stage ((F=4.423, P=0.012). Moreover, the overexpression of Tob was significantlycorrelated with lymph node metastases. However the expression of Tob wasno correlation with the age, gender of patients and pathologic type ofcolorectal cancer in this study.CONCLUSION: The up-regulation of Tob may be closely associated withtumorigenesis and metastasis of colorectal carcinoma.
|
PDF Full Text Request