Expression Of CK19-mRNA In Peripheral Blood Of Lung Cancer Patients By Real-time Fluorescent Quantitative RT-PCR And Its Clinical Significance

Objective To investigate the expression of CK19 mRNA in peripheral bloodcells in patients with non-small cell lung cancer and its clinicalsignificance.Methods The expression of CK19 mRNA in peripheral blood cells of 35patients with non-small cell lung cancer and 10 normal healthy volunteerswere detected by real-time fluorescent quantitative RT-PCR.Results The positive expression rates of GAPDH mRNA of 35 patients withnon-small cell lung cancer and 10 normal healthy volunteers in peripheralblood cells were 100%.24 patients were detected the positive expressionof CK19 mRNA in 35 patients with non-small cell lung cancer, The positiverate was 68.6%. Of these 10 normal volunteers, none was positive for CK19mRNA. The positive expression pf CK19 mRNA in peripheral blood was notrelated to the clinical staging, phathological type, and tumor celldifferentiation, but was closely related with the lymph node metastasisstatus.Conclusions Detection of the CK19 mRNA expression in peripheral blood byreal—time fluorescent quantitative RT—PCR has a higher sensitivity andspecificity in patients with non-small cell lung cancer. It may help toearly diagnose blood-berne metastasis for patients with lung cancer andevaluate the cancer more corrrectly and make the best treatment plan.