Investigation Of The Anti-tumor Activity Compounds On Aralia Elata (Miq.) Seem.

in order to find out the anti-tumor activity compounds, we have investigated thestem of the root bark of Aralia Elata (Miq.) Seem. from Badao in Yanji city. We made ethyl acetate and n-butanol extracted fraction from ethanol total extracted fraction on root bark of Aralia Elata (Miq.) Seem. Five compounds have been obtained by means of silical gel column chromatography repeatedly and reversed preparative HPLC and preparative TLC. On the basis of detailed analysis of the spectral data (such as ~1H-NMR,~13C-NMR,APCI-MS ets.) and chemical method, five compounds were elucidated together. These compounds are as following: Stigmasta-5, 22-dien-3-ol (CJM-1), oleanic acid (CJM-2), scoparone (CJM-3), stigmasta-3-O-glu-copyranoside (CJM-4), 3β-hydroxyl-cholic acid methyl ester.Among them, Compounds CJM-1, CJM-4 and CJM-5 were isolated in first time from this plant.In vitro against human tumor cell lines (MTT), we didn't find anti-tumor activity on each extract fractions from the ethanolic extract and five compounds we obtained. But the water extract fraction and 75% ethanolic liquid extract fraction from macroreticular resin have strongly anti-tumor activity. And the specific structures of these compounds still need to be investigated farther.