The Studies On MCP: Preparation, Analysis And Determination And Its Anti-tumor Effect

Pectin is a ubiquitous biological macromolecular in plants, which has complexbioactivities and functions. Polysaccharide derivative--MCP, which has anti-tumoreffect, was obtained by modifying citrus pectin.Polysaccharide MCP-1 was obtained through pH-modifying low-methylated citruspectin. The same method was used to prepare MCP-2 with high-methylated citruspectin. MCP-1 and MCP-2 were analyzed and determined by phenol-sulfuric acid,m-hydroxyldiphenyl, volumetry, HPGPC,agarose gel electrophoresis, IR and UVmethods.The results of MCP-1 analysis were following: the total sugar content in MCP-1 was98.1%, uronic acid content was 81.0%, and methoxy group content was 0.15%. Itshomogeneity was tested by agarose gel electrophoresis and HPGPC. Its Mw was37373 and PD was 1.86 by HPGPC analysis. The results of MCP-2 were following:the total sugar content in MCP-2 was 99.2%, uronic acid content was 84.1% andmethoxy group content was 0.92%. Its homogeneity was tested by agarose gelelectrophoresis and HPGPC. Its Mw was 35968 and PD was 1.84 by HPGPC analysis.The results of IR showed a typical polysaccharide absorbent pattern. No obviousabsorbance of protein and other substances was showed in the scanning range of UV.The anti-tumor effects of MCP were tested in mice with three transplanted tumors:sarcoma S180, cervical carcinoma U14 and hepatocarcinoma H₂₂. MCP-1 significantlyinhibited tumor growth of H₂₂ cell in high dose group;the inhibition rate was 47.8%.In 4 mg/kg and 2 mg/kg groups, the growth inhibitory rates of MCP-1 against the U14cell were 36.5% and 38.51%, respectively. MCP-1 had no anti-tumor activity on theS180 cell. MCP-2 showed no significant inhibition against tumor growth of U14 cell.MCP-1 and MCP-2 exhibited no cytotoxicity to the cell lines of human brain gliomaTJ-905 and human cervical carcinoma HeLa by MTT assay.