Research Of Frozen Tissue Microarray And The Biological Significance And Expression Of Skp2 And Related Protein In Tissue Microarray

Objective:We studied the facture and its feasibility on frozen tissue microarray using mold doing by ourselves, so that we could provide a fast, simple and economical technique for scientific study. Material and Method:All samples in our research were collected from the General Hospital of Tianjin Medical University, the First Central Hospital and the Chest Hospital in Tianjin from 2004 to 2005. A total of 48 dots frozen tissue microarray were constructed in this study including primary lung cancer 19 samples and normal lung tissue 5 samples from primary lung cancer. Haematoxylin—eosin and immunohistochemical stains were performed on the sections and we assess the feasibility on morphology and genetic expression. Result:1. OCT block of frozen tissue microarray and HE-stained: The cores on frozen tissue microarray were regularly arranged and had no shift and distort. After HE-stained, morphology of the cores was well preserved. They were answered for the request of research.2. Immunohistochemistry of array slides: we performed immunohistochemistry on the tumor tissue microarray with antibodies for EMA. EMA staining is uniform across the sample and gives the expected cytochylema-associated staining. There is no background staining.3. We compared the staining of Immunohistochemistry on section of frozen tissue microarray to the results on normal slides. The results showed the accord rates were 94.7% (18/19) , and there were no significant differences between them each other (p>0.05) .Conclusions:1. The method of frozen tissue microarray is feasible. HE-stained and immunohistochemical stains showing this methodology will be useful for morphology and immunohistochemical based protein analyses.2. Frozen tissue microarray is a new technique which has characters of high-throughout, economy and simple. It will be broadly applied in study fields.3. When combining frozen tissue microarray technique to immunohistochemical method, taking 2 cores (diameter is 1.5mm) from the whole-tissue sections was an accurate and economical method.