Expression And Significance Of Skp2 And P27^kip1 Proteins In Bladder Transitional Cell Carcinomas By Using Tissue Microarray Technique

Objective: To investigate the expressions and correlations of Skp2 and P27kip1 in bladder transitional cell carcinomas (BTCC) tissues and nomal bladder tissues and their preliminary clinical significance.Methods: Expressions of Skp2 and P27kip1 were detected in 76 cases of BTCC tissues and 22 cases of nomal bladder tissues by using tissue microarray technique and immunohistochemistry test (S-P method). The relationship of Skp2 and P27kip1 expression with clinicopathological features and prognosis in BTCC was then analysed. The correlation between Skp2 and P27kip1 was also studied.Results:1. Skp2 expressed mainly in nucleus. The positive rates of Skp2 expression was 51.32% in BTCC tissues, remarkably higher than 13.64% in nomal bladder tissues, which differences were statistically significant (P<0.01).2. The positive rates of Skp2 protein expression in BTCC became higher when tumor differentiation degree went poor. The positive rates of Skp2 protein expression in well, moderately and poorly differentiated carcinomas were 23.81%, 56.41%, 75.00% respectively, the difference was significant in statistics (P<0.01).3. The expression of Skp2 protein in BTCC had significant relationships with tumor clinical stages, lymph node metastasis and relapse of the tumor, which difference exists statistical significance (P<0.05), whereas no relationships with gender, ages, tumor numbers and sizes (P>0.05).4. 5-year survial rate of BTCC in positive Skp2 expression group (46.2%) was remarkably lower than that of Skp2 negative expression group (75.7%,χ2=7.195, P=0.007).5. P27kip1 expressed in nucleus and cytoplasm. The positive rates of P27kip1 expression was 44.74% in BTCC tissues, remarkably lower than 95.45% in nomal bladder tissues. The difference between them exists statistical significance (P<0.01).6. The positive rates of P27kip1 protein expression in BTCC became lower when tumor differentiation degree went poor. The positive rates of P27kip1 protein expression in well, moderately and poorly differentiated carcinomas were 71.43%, 35.90%, 31.25% respectively, the difference was significant in statistics (P<0.01).7. The expression of P27kip1 protein in BTCC had close relationships with tumor clinical stages, lymph node metastasis and relapse of the tumor, which difference exists statistical significance (P<0.05), whereas no relationships with gender, ages, tumor numbers and sizes (P>0.05).8. 5-year survial rate of BTCC in negative P27kip1 expression group (47.6%) was remarkably lower than that of P27kip1 positive expression group (75.8%,χ2=6.492, P=0.011).9. Skp2 expression in BTCC had negative correlation with P27kip1 expression ( rs=-0.843,P<0.01). The negative correlation between Skp2 and P27kip1 was observed also in nomal bladder tissues ( rs=-0.500,P<0.01).10. The survial rate of BTCC patients with both Skp2 positive expression and negative expression of P27kip1 was significantly lower than that of other cases.Conclusion:1. The large-scale application of tissue chip is viable to test the clinical specimens efficiently. It shows that the method is save-timed, convenient, economy and accurate. It met the requirements of researches on bladder carcinomas.2. The expressions of Skp2 and P27kip1 proteins in BTCC had remarkable relationships with malignant behaviors such as tumor poor differentiation, high clinical stage, lymph node metastasis and relapse of the tumor. Skp2 and P27kip1 can be used as the independent indicators for the prognosis of BTCC.3. Over expression of Skp2 along with down-regulation of P27kip1 may indicate a tumor with higher invasive tendency and poor prognosis. Combined detection of the expressions of Skp2 and P27kip1 had an important value in the evaluation of malignant potential and prognosis of BTCC.