Research Of Biological Significance And The Molecular Mechanism Of OPN And Relative Protein Expression In Lung Cancer Progression Tissue Microarray

Objectives:We intended to detect the expressions of OPN, CD44v6 and MMP-9 protein in lung cancer progression tissue microarray and to discuss their roles in lung cancer genesis and progress and analyze the probable molecular mechanism. With these results, we wanted to put our discoveries into the clinicopathological diagnosis, prognosis evaluation and gene therapy.Methods:We used 2-step plus Poly-HRP Anti—Mouse/Rabbit IgG immunohistochemistry method to detect the expressions of OPN, CD44v6 and MMP-9 protein, in tissue microarray with 270 cores, in which there were 89 cases of primary lung cancer, 12 cases of lymph node metastasis of lung cancer, 12 cases of precancerous lesion and 10 cases of normal lung tissue, analyzed the relationship between they and clinical parameters. All data were processed by SPSS version 11.0 analysis software. Results:1. In primary lung cancer, the expression degrees of OPN protein had significantly positive correlation clinical stages of lung cancer (p<0.05). The positive rate of OPN protein was significantly higher in the group with lymph node metastasis than that without lymph node metastasis (p<0.05) ; it was higher in the group of III+IV stage than inⅠ+Ⅱstage (p<0.05) .2. The positive rate of CD44v6 protein was significantly related lymph node metastasis and clinical stages. It was higher in the group of high-middle differentiation than that of low-un differentiation; it was higher in the group without lymph node metastasis than that with lymph node metastasis (p<0.05) ; it was higher in the group ofⅠ+Ⅱstage than that inⅢ+Ⅳstage (p<0.05) .3. The positive rate of MMP-9 protein was significantly related to lymph node metastasis and clinical stages. It was higher in the group with lymph node metastasis than that without lymph node metastasis (p<0.05) ; it was higher in the group ofⅢ+Ⅳstage than inⅠ+Ⅱstage (p<0.05) .4. There was significantly correlation between OPN and CD44v6, There was significantly correlation between OPN and MMP-9, there was significantly positive negative between CD44v6 and MMP-9.Conclusions:1. The expression of OPN was higher in premalignant lesions and lung cancer samples ,in normal lung tissues its expression was 0%,which suggested it involved in carcinogenesis of human lung cancer. We detected that the expression was much higher with lymph node metastasis It proved that OPN protein had a relationship with metastasis function in lung cancer. The OPN expression correlated with gross types of lung cancer, and preferred to expression in central type .Our study provided theoretical basis to the individual therapy of lung cancer. There were some differences among the OPN expression of different types of lung cancer. Though the differences were not significant, it still could prove the mechanism in different types were various.2.The positive rate of CD44v6 protein was higher in primary lung cancer than that in normal lung tissue and precancerous lesion, which suggested that CD44v6 promote the genesis and progress of lung cancer.3.The positive rate of CD44v6 protein was significantly related to lymph node metastasis and clinical stages, which suggested that CD44v6 can play along with infiltration and metastasis of tumor through promoting tumor proliferation and vascularization; it can be a reference marker of prognosis evaluation.4.The positive rate of MMP-9 protein was higher in primary lung cancer than that in normal lung tissue and precancerous lesion, which suggested that MMP-9 promote the genesis and progress of lung cancer.5.There was no significant relationship between CD44v6 and MMP-9,which suggested they might have different mechanism on depressing the apoptosis of tumor cells and inducing tumor angiogenesis. They played different roles in carcingenesis and growth of human lung cancer.