The Study Of Expression And Clinical Significance Of Skp2 And P27～(Kip1) In Ovarian Tumor

Objective:p27^Kip1 is a kind of cyclin-dependent kinase inhibitors, reduced levels of p27^Kip1 are associated with many malignant tumors, p27^Kip1 is viewed as a potent prognostic predictor of reduced survival of cancer patients. S-phase kinase associated protein 2(Skp2), an F-box protein, is required for DNA replication. Skp2 recognize specific p27^Kip1 and makes it ubiquitination and degradation. The findings suggest that overdegration of p27^Kip1 might be attributed to Skp2 overexpression. The purpose of this study is to examine the expression and correlation of Skp2 and p27^Kip1 in ovarian turmors, as well as their clinical significance.Method:Immunohistochemical technique is used to evaluate the expression of Skp2, p27^Kip1 in 25 ovarian carcinomas, 10 borderline ovarian tumors and 15 benign ovarian tumors respectively. The potential correlations of the two proteins expression with the clinical pathologic stage, pathologic grade and histological classification are analyzed as well.Results:1. The positive expression of p27^Kip1 and Skp2 are detected in cellular nuclei of ovarian tumors.2. p27^Kip1 expressions are detected in benign ovarian tumors, borderline tumors and carcinomas and the positive rates of p27^Kip1 are 80%, 60% and 36% respectively.expression is not detected significantly more often in benign tumors (PO.05) and carcinomas (P<0.05) than in borderline tumors, while a significantly higher detection rate is detected in benign tumors than in carcinomas (PO.05).3. Skp2 expression isn't detected in benign ovarian tumors, but is detected in borderline tumors and carcinomas, and the positive rates are 40% and 88% respectively. A significantly higher detection rate of Skp2 expression is observed in borderline tumors than in benign tumors. Skp2 expression is detected significantly more often in carcinomas than in benign tumors (PO.05) and borderline tumors (PO.05).4. The rates of p27Epl detection are not different between serous groups and non-serous groups (P>0.05). A significantly higher detection rate of p27KipI expression is observed in early-stage diseases compared with advanced-stage diseases (PO.05). A significantly higher detection rate of p27Kipl expression is observed in high histological grades than in low histological grades of the tumors (PO.05). Lymph node metastasis group compared with non-lymph node metastasis group, the expression of p27Kipl is different (P<0.05), while p27Kipl expression has no relation with age.5. The frequency of Skp2 expression in advanced-stage diseases is much higher than in early-stage diseases (PO.05). A significantly higher detection rate of Skp2 expression is observed in low histological grades than in high histological grades of the tumors(P<0.05). The rates of Skp2 detection are not different between serous groups and non-serous groups (P>0.05). The expression of Skp2 has no relation with age and lymph node metastasis.6. hi 25 cases of ovarian carcinomas, there are 22 cases of carcinomas Skp2 protein expression positive, and 6 of which are p27Kipl expression positive, 16 ofwhich are negative, the positive rate is 27.3%(6/22);3 cases of Skp2 negative expressions, the expressions of p27Kipl are all positive, through statistics analysis, there is difference between Skp2 and p27Kipl expression (P<0.05), Spearman analysis shows there is a significant negative correlation between Skp2 expression and p27Kipl expression.Conclussions:1. The expression of p27Kipl protein in ovarian carcinoma is lower than in benign tumor, and is weaker in advanced-stage diseases and low histological grades, which shows decreased expression of p27Kipl might play an important role in the development and progression of ovarian tumors. p27Kipl protein expression is lower in lymph node metastasis group, which shows p27Kipl may play a role in the metastasis of ovarian carcinoma.2. The expression of Skp2 in ovarian carcinoma is higher than in benign tumor and borderline tumor, and in ovarian carcinoma the expression are higher in advanced-stage diseases and low histological grades, which shows Skp2 overexpression might play an important role in the development and progression of ovarian tumors. Skp2 protein overexpression should be an independent prognostic factor for ovarian carcinoma.3. Skp2 overexpression is significantly associated with reduced p27Kipl expression in ovarian carcinomas, which shows probable relation with bad prognosis of patients.