To Study The Significance Of Multi-cytokines In Various People By Using Biochip Technology

Knowledge of the complexity of the cytokine network and the role played by cytokines is critical in understanding normal and pathological processes. Therefore assaying a number of cytokines in the one sample has become of increasing interest in laboratory medicine. The knowledge gained from multiple cytokine analysis should allow better diagnosis and disease management. In this study we detected 12 serum cytokines of various people group by using biochip technology, and the results showed us that cytokines played a very important role in human body.1. The EVIDENCE 180 analyser is a fully automated biochip array system. The cytokine panel can quantitatively test for IL-1α , IL-1β , IL-2, IL-4, IL-6, IL-8, IL-10, VEGF, IFN γ , EGF, MCP-1 and TNF α simultaneously. Serum cytokines is very stable on healthy population and it is no correlations with ages and gender. It is very useful to evaluate the functions of body's immune system.2. The dynamic studies on the serum cytokines level of different stage in the diagnosed SARS patients. Compared with healthy group, IL-6 , IL-8 , IL-10 , IFN γ increased obviously and IL-1 α, IL-2, IL-4, VEGF, EGF, MCP-1, TNF a decreased obviously. Whereas IL-1β has no statistic changes among different stages of SARS. There were obvious changes of multiple cytokines in different phase of SARS pathological process, especially in the early phase. It is further support the hypothesis that over-reaction of the immune system initiated the pathological injuries of the patients.3. Compared with healthy population EGF is lower obviously. After heavy intensity training of 5 weeks IL-8, VEGF, TG, CH, HDL, ALB, GLU, Na,K and CL decreased obviously and EGF > Ua increased obviously. Nanometer-particulate LiGuYuan capsule can influence serum IL-1 P > IL^ EGF> TG^ GLU evidently. Heavy intensity training caused immune ability decreased and nanometer-particulate LiGuYuan capsule can anti-fatigue and increase the capability of body's respond.4. In the patients who after kidney transplantation and treated by FK506, the serum IL-6, IL-8 and IL-10 increased obviously, whereas serum EGF decreased markedly. The immune function of the patients after kidney transplantation was still abnormal and serum cytokines were very useful to follow up and monitoring the rejection of the transplanted organ in them.5. The dynamic studies on the variation of serum cytokines and biochemical parameters in post kidney transplanted patients, we found serum IL-6 and IL-8 had reached the highest level at 7 days after kidney transplantation, whereas IL-2 and IL-10 were reached the highest level at 14 days. Then they decreased gradually. The decreased VEGF, EGF and MCP-1 in kidney failure patient had increased after transplantation. UN, Cr, Ua and Glu had been restored to normal level after transplantation and there was obviously relationship between IL-8 and serum LDH. The variation of serum cytokines were related with the recovery of kidney function and tissue injury, it is a potential useful marker to follow up organ transplantation patients.6. In the stable stage of COPD, the level of serum IL-8 is increased and it is possible involved the processes of chronic airway inflammation.Based on these studies, there were very important significance of multi-cytokines in various patients, especially in the patients with inflammation disease and immune-disorder. So that, to detect various serum cytokines associated is very useful to diagnose and monitor the multi-disease.