Experimental Studies On The Biomedical Applications Of Magnetic Nano-paticle

Purpose: The wish of the work is to study the effect of protein selection from several traditional Chinese medicine with MNP-NH2. And the mechanism of tumor cell' s apoptosis induces by Singseng protein. Method: l.As a result of the reaction with AEAPS, magnetic nanoparticle was modified and joined -NH₂ on its surface. This material was called MNP^NH₂ as a whole. MNP-NH₂ can be used in selection of protein. 2. we used Methyl Thiazolyl Tetrazblium colorimetic and colony formating method to obervate the effect of CFU-GM and cell multiplication of K562 tumor cell induced by ginseng protein 3. MFM and DNA gel-electrophoretic apparatus also was used to test the occurrence of tumor cell' s apoptosis induced by ginseng protein and MNP. Result:The result suggests that the combine betweenMNP-NH2 and BSA has a linear correlation(r=0.9942, p<0.001). Forethmore, several proteins from different traditional Chinese medicineswere selected by MNP-NH2. Compared with the control groups, the results of experiment groups have significant difference(P<0.05—0.001). This research indicated that MNP-NH₂ can be extensively used in protein selection. Meanwhile, this is a effective new way to realized the modernization of traditional Chinese medicine.2, Ginseng protein can induce K562 tumor cell' s apoptosis and there was significant difference compared with control group (P<0.01), inhibition ratio had significant positive correlation with medication dosage and time (r=0. 974) . Ginseng protein can promote the growth of CFU-GM too. And there was significant difference compared with control group also(P<0. 01). Conclusion:MNP can be used in seperation of protein and mRNA effectively. Ginseng protein can induce K562 tumor cell' s apoptosis and promote the growth of CFU-GM. MNP which was modified with -SH and joined antisense oligodeoxynucleotideson its surface can be swallowed by K562 tumor cell and induced apoptosis of K562.