The Chemical Constituents Of Marine Alga Ulva Pertusa Kjellm

Ulva pertusa kjellm is an edible macroalgae of Chlorophy. It contains rich nutrient substance such as protein, polysaccharides, vitamin, mineral element etc., which is good for body's growth, development and health. There are also high value of the medical application of it. Several ancient medical books recorded that U. pertusa had many traditional active properties, and it was widely used in region of china, associated with other medicial plant, to treat for goiter, hypertension, oedema, laryngitis, cough, and so on. The present studies of chemical constituents and pharmacological effect about the U. pertusa are scare, so the usage of the plant is insufficient. Now more and more attention has given to it. According to recent studies, it has been shown significant biological activities, such as antitumor, antivirus, antibiosis, anticoagulated blood, antiaging and lowering of blood fat and cholesterol, and so on. Therefore the paper was to investigate the chemical constituents of this green algae systematically. From the plant, 35 compounds were isolated by repeated on silica, RP-18, sephadex LH-20, macroporous resin column chromatography, and 28 compounds were identified by various spectroscopic methods (UV, MS, ¹H-NMR, ¹³C-NMR, DEPT, COSY, HSQC and HMBC). Compound 10, 12 and 20 were new compounds;Compound 1 was a new natural product and its spectra data was the first report here;Others were isolated from Ulva for the first time except the compounds 9, 15,17,22 and 28.The structures of above 28 compounds from Ulva pertusa were listed as follows: (1). Phenolic compounds1. (2,4-dichlorophenyl)-2,4- dichlorobenzoate(l)2. N-phenyl-2-naphthylamine(2)3. 5-hydoxy-3, 6, 7,3', 4'-pentamethoxyflavone(3)4. 5-hydoxy-6, 7, 3', 4'-tetramethoxyflavone(4) 5.p-ethoxybenzoic acid(5)6. Cis-asarone (6)7. Trans-asarone (7)8. y-asarone(8)(2). Compounds of SteroidI.j3-sitosterol(9)2. 24, 28-diaminocholesterol(10)3.24-hydrooxy-24-vinyl-cholesterol(l 1)4. 24-ethylperoxy-24-vinyl-cholesterol(12)5. 29-hydroxystigmasta-5,24(28)-dien-3-ol(13)6.29-hydroperoxystigmasta-5,24(28)-dien-3-ol(14)7. Daucosterol(15)(3). Compounds of triterpene1. a-amyrin(16)2. Ursolic acid(17)3. Maniladiol-3-o- nonadecanoate (18)4. 22-α-methoxyfaradiol 3-o-nonadecanoate (19)5. 22-a-ethylfaradiol 3-o-nonadecanoate (20)6. Faradiol 3-o-nonadecanoate(21) (4). Compounds of diterpene 1.7>fl/w-phytol(22)2. Phytyl-palmitate(23)3. Phytyl-acetas(24)4. Isophytol(25)(5). Compounds of monoterpenel.Menthol(26)(6). Straight chain compounds1. Docosanol(27)2. Palmitic acid(28)...