Study On Extraction And Bioactivities Of Polysaccharides From Ulva Pertusa Kjellm

Ulva pertusa Kjellm is a large economical algae which contains abundant nutriment such aspolysaccharides, protein, amino acid, vitamin, inorganic mineral element. It possesses thebioactivities which can be helpful in the process of antitumor, antivirus, hypoglycemic,antihyperlipidemic and antioxidation. So it plays a very important role in facilitating the normalgrowth and development as well as ensures the health development of the organism. In our study,we analyzed the method in which water-solubility polysaccharides could be extracted and itseffects on hypoglycemic, antihyperlipidemic and antioxidation from U. Pertusa.We used hot water extraction process to extract U. pertusa polysaccharides. The bestextraction conditions obtained by orthogonal test using the water addition, extractiontemperature and extraction time as changing factor （the water addition:40times; temperature:125℃; time:4h）. And the final results showed that the leaching rate of U. pertusapolysaccharides was20.22%and the total sugar content was49.65%.This paper researched the hypoglycemic effects which U. pertusa polysaccharides can haveon the hyperglycemic model mice. We used the method of injecting alloxan to the peritoneum tobuild the hyperglycemic model and later giving U. pertusa polysaccharides to the normal andhyperglycemic model mice respectively intragastric administration. The results showed thatdifferent dose of U. pertusa polysaccharides [250mg/（kg·d）、500mg/（kg·d）、1000mg/（kg·d）]could obviously decrease the glucose concentration in serum of hyperglycemic mice that wasinduced by alloxan and will not decrease the glucose concentration of normal mice. Also thedecrease of glucose concentration in hyperglycemic model mice was more obvious with theincrease of the injection of U. pertusa glucose. Finally, certain dose of U. pertusa glucose[1000mg/（kg·d）] plays the same role as metformin in decreasing blood glucose concentrationcausing hyperglycemic mice by alloxan.This paper analyzed the effects of antihyperlipidemic by U. pertusa polysaccharides in themodel of hyperlipemia mice. We used the method of feeding high fat diet to mice in order tobuild the hyperlipemia model mice and givving U.pertusa polysaccharides intragastricadministration. The final result showed that different dose of U. pertusa polysaccharides couldall decrease the content of the total cholesterol（TC）, triglyceride（TG） and low density lipoproteincholesterol（LDL-C）, and increase the content of high density lipoprotein cholesterol（LDL-C）together with the ratio of high density lipoprotein cholesterol to the total cholesterol （HDL-C/TC） in hyperlipemia model mice. However, it has no obvious effects on normal mice. High dosegroup [1000mg/（kg·d）] has a similar effect on hyperlipemia model mice compared to simvastatin,while the effects of increasing the ratio of high density lipoprotein cholesterol to the totalcholesterol （HDL-C/TC） were better than that of simvastatin.This paper also analyzed the antioxidation effects of U. pertusa polysaccharides. The studyusing antioxidation model in vitro showed that U. pertusa polysaccharides has a directscavenging effect on superoxide anion radical （O₂^-·） and hydroxyl radical （·OH）. And thescavenging effect increases with the increase of the concentration of polysaccharides. Also U.pertusa polysaccharides has a strong inhibition effect on lipid peroxidation. And it showed acertain dose-effect relationship between inhibition effect and the concentration ofpolysaccharides. Finally, U. pertusa polysaccharides has an effect on the antioxidation to lipid,and the inhibition effect increases with the increase of the dose.