Screening Effective Parts Of Ulva Pertusa Polysaccharide And Polysaccharide-Fe(Ⅲ) Complex For Its Anti-Radiation Effects

coast, and its resources are very rich. Ulva pertusa has a high economic and medicinal value, contains protein, amino acids, sugars and other nutrients,who is very delicious.The polysaccharide of Ulva pertusa can enhance immunity, with the proposed anti-blood, anti-tumor, reduce cholesterol, anti-oxidant and other medical support functions.The polysaccharide of Ulva pertusa was extracted by water,and precipitated with ethanol, then separated by centrifuagation, the crude polysaccharide extract was obtained. After pretreatment, the polysaccharide of Ulva pertusa was futher purified by 100ku and 30ku membrane, and then the polysaccharide of Ulva pertusa was divided into different molecular weight polysaccharide solution(≧100ku,30~100ku and≦ 30ku). The radioprotective ability of different molecular weight polysaccharide solution were studied using micronucleus assay of mice bone marrow cells, and determination of the spleen index, the activity of SOD and the level of malondialdehyde (MDA). Eventually found the polysaccharide solution less than 30ku showed best ability of radioprotection, it reduced the radiation induced frequency of micronuclei(P<0.01), and increasing the spleen index (P<0.05) and the activity of SOD in the liver after irradiation in mice significantly. The radiation-induced decrease of MDA in the liver by pretreatment wtth polysaccharide (≦30ku). The results show that polysaccharide(≦30ku) possess effitive antiradiation activity.The polysaccharide of Ulva pertusa(≦30ku) and Fe(Ⅲ) chelation,and got Ulva pertusa polysaccharide iron complex. Result showed high iron(27.4%) of it by atomic spectrophotometry. And in the 850cm-1 and 650cm-1 Office had characteristic peaks by infrared spectroscopy, indicating its iron core wasβ- FeOOH. And also, Ulva pertusa polysaccharide iron complex possess effitive antiradiation activity,compared to the polysaccharide of Ulva pertusa, and the difference of them were not significant.2-DE study of spleen in mice radiation experiments fed polysaccharide and polysaccharide iron (Ⅲ) show that,compared to control group , 2 proteins are 2-fold up-regulated and 13 2-fold down-regulated in irradiation control group, and compared to irradiation control group , there are 14 2-fold up-regulated proteins , 5 2-fold down-regulated in polysaccharide group and 12 2-fold up-regulated proteins,8 2-fold down-regulated in polysaccharide iron group including 78kDa glucose regulated protein, prolyl hydroxylase beta, subunit calreticulin precursor, micro- tubulinβ5 and vimentin which all were associated with immune and injury.