| Background and Objections. MCP-1 is one of chemokines synthesized by monocytes, endothelial cells and et al. MCP-1 is associated with the increase synthesis of ECM and the accumulation of FN, as well as involving in the process of inflammation. In vitro experiments have shown that high concentration of glucose induced MCP-1 and TGF- β secretion of peritoneal mesothelial cells in non-infective conditions, by which, we suspect, can lead to peritoneal sclerosis. In the present study, we comprehend the changes of expression of MCP-1 mRNA of PMC and accumulation of FN in parietal peritoneum by different concentrations of PDF in short-term period through the rat CAPD models, and then to investigate the effects of PDF on MCP-1 produced by PMC in non-infection situations and the associations with CAPD related peritoneal sclerosis.Methods. The study was performed on 32 male S-D rats weighting between 250g and 300g. The rats were randomly divided into 4 groups (n=8) and injected intraperitoneally 40ml NS as control, 1.5%, 2.5% and 4. 25% PDF( Dianeal PD-2, Baxter) into the respective group daily for 2 weeks. On the 15th day, after a 4-hour dwell, the rats were put to death, dialysate samples and serum were collected to measure the MCP-1 protein levels using an ELISA kit, parietal and visceral peritoneum tissue were taken to determine the MCP-1 mRNA expression by ISH and the FN accumulation by ICH.Results. The MCP-1 protein levels of PDF were increased in 4. 25%group compared with that of 1. 5% and 2. 5% groups, but the difference had no statistic significance, while the levels in NS control group were significantly increased compared with the other 3 experimental groups (F = 4.890, P= 0.007). The MCP-1 protein levels of serum in the whole 4 groups showed no significant difference (F=0.763, P=0. 524). In the NS group, the MCP-1- mRNA expression of PMC was increased compared with that of 4. 25% group, and the values of 4. 25% group were higher than that of 1. 5% group and 2. 5% group, and the differences between groups were statistically significant (F=8. 564, P=0. 001). Also in the NS group, the FN levels of parietal peritoneal tissue were the highest of the 4 groups, and the second was 4.25% group which values were higher than that of 1. 5% group and 2. 5% group, but the differences between groups had no statistic significance (F=0. 640, P=0. 590). We also do the correlation analysis between FN and MCP-1 of PDF, and FN and MCP-1 mRNA, and found positive correlation (r= 0.613, P=0. 002;r=0. 438, P=0. 042).Conclusions. ?PDF could induced the increase of MCP-1 mRNA expression of rat PMC in short-term exposture, and the increase followed by the increase of glucose concentrations of PDF, while NS could accelerated the synthesis of MCP-1 mRNA. (2) NS could induced the MCP-1 synthesis more intensively than PDF(Baxter PD-2), that suggested the most serious damage of peritoneum may be found in NS group, this is coincident with Styszynski's result. (3) In the serum, the MCP-1 protein levels were lower than that of PDF, that suggested some other sources of MCP-1 exist except PMC, such as marophage. ?In short-term exposture, NS and PDF of different glucose concentrations had no significant effects on the components of ECM, such as FN. So we can conclude that high glucose concentration PDF(4. 25%) had no effect on peritoneal structure in short-term CAPD. (5) Accumulation of FN was associated with the increase expression of MCP-1, so we can conclude that the increase expression of MCP-1 may be one of the reasons induced CAPD relative peritoneal sclerosis.
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