The Study Of PTEN P16 And P53 Protein Expression In Uterine Cervix Carcinoma

Objective To study the expression of PTEN, p16 and P53 proteins in cervical carcinoma and to analyze their relationship with clinicopathologic features.Methods Using SP immunohistochemical technique,we examined the expression of PTEN, p16 and P53 in 10 normal cervical tissues, 10 cervical atypical hyperplasia, 8 carcinoma in situ and 46 invasive carcinoma of cervix uteri.Results The positive expression rates of PTEN,P16 and P53 proteins were respectively 100% , 0%, 0% in 10 normal cervical tissues, 90%, 20% , 10% in cervical atypical hyperplasia, 50%, 50%, 62.5% in carcinoma in situ and 28.26%, 78.26%, 89.13% in cervical carcinomas. The positive rate of P16 and P53 in cervical carcinomas was respectively much higher than in normal cervical tissue and in cervical atypical hyperplasia (p<0.01). but the positive rate of PTEN in cervical carcinomas was much lower than in normal cervical tissue and in cervical atypical hyperplasia (p<0.01), The expression rates of PTEN,P16 and P53 proteins ,respectively is not a significant different in carcinoma in situ and cervical carcinoma.There was a significant correlation between the expression of P16 and p53 in invasive carcinoma of cervix uteri(p<0.05). Abnormal PTEN expression was related to tumor size and stage of the disease, lymph node metastasis.There were no statistically significant differences in the P16 and P53 status with respect to age of patients, tumor length, histologicdifferentiation and clinical stage of the disease. In invasive carcinoma of cervix uteri there was not related to lymph node metastasis and expression of P16 and P53 gene.Conclusions The loss of PTEN expression is closely related to tumorigenesis and development of the cervical carcinomas ,and overexpression of tumor suppressor p16 and P53 occurs in early phase of the cervical cancer.PTEN may play an important role in invasion and metastasis in human cervical carcinoma.