The Clinical Significance Of The Expression Of MMP-9 And The Infiltration Of TAMs In Squamous Carcinoma Of Cervix

Objective:To study the relationship between the expression of MMP-9(Matrix metalloproteinase-9)and the number of TAMs(Tumor-associated macrophages)in squamous carcinoma of cervix(SCC)and clinicopathlogic characteristics of SCC.It is expected to provide experimental evidence and theoretical basis for the occurrence,development and prognosis of SCC.Methods:We collect around 100 cases of cervical squamous cell carcinoma patients'specimen,who were performed extensive hysterectomy and pelvic lymph node dissection and make a definite diagnosis of by pathological examination,dated January 2012 till January 2016 in The People's Hospital of Hechuan district.In the control group,there are 60 specimens of CIN lesions(Including 20 cases of CINI,CIN II and CIN III)and 20 cases of normal cervix specimens were selected.All of the above cases were not treated with radiotherapy and chemotherapy before operation,and no complications such as diabetes,hypertension and kidney disease were found.Immunohistochemistry was used to examine the expression of MMP-9 and CD68 in SCC,CINIII,CINII,CINI andNormal cervical tissues.TAMs were labeled with CD68,and the number of TAMs was counted.SPSS 19 statistical software was used for statistical analysis.Results:1.The expression of MMP-9 in CINI-III:There were 2(10.0%)cases of high expression of MMP-9in the 20 cases ofCINI;There were 6(30.0%)cases of high expression of MMP-9in the 20 cases of CINII;There were13(65.0%)cases of high expression of MMP-9 in the 20 cases of CINIII.The difference of MMP-9 expressionamongthe CINI and CINII tissue has not statistical significance(?~2=2.50,P>0.05);The difference of MMP-9 expressionamongthe CINI and CINIII tissue has statistical significance(?~2=12.81,P<0.05);The difference of expression among the CINII and CINIII tissue has statistical significance(?~2=4.91,P<0.05).2.The differential expression of MMP-9 in different cervical tissues:There were 88(88.00%)cases of high expression of MMP-9 in the 50 cases of SCC;there were 21(35.0%)cases of high expression of MMP-9 in the 60 cases of CIN,There are 1 cases of high expression of MMP-9 in 20 cases of normal cervical tissues;The difference of MMP-9 expressionamongthe SCC and CIN tissue has statistical significance(?~2=31.69,P<0.05);The difference of MMP-9 expressionamongthe SCC and normal cervical tissue has statistical significance(?~2=42.86,P<0.05);The difference of MMP-9expressionamongthe CIN and normal cervical tissue has statistical significance(?~2=6.77,P<0.05).3.The expression of MMP-9 in SCC tissues and the clinical and pathological features of SCC:The expression of MMP-9 is closely connected with patients'clinical stages,pathological grading and lymphatic metastasistransferand;The expression of MMP-9 in cases of clinical stage II,lymph node metastasis and invasion depth more than 1/2cervical wall was significantly more than the cases of clinical stage I,no lymph node metastasisthere and invasion depth less than 1/2 cervical wall,Differences are statistically significant(P<0.05);however,The expression of MMP-9 has not relation with patients age,tumor size and degree of tumor differentiation.4.The number of TAMsin CINI-III tissues:The number of TAMs is4.41±1.07?6.31±1.39?17.29±4.36/HP in CINI,CINII and CINIII;The difference number of TAMs among the CINI and CINII tissue has statistical significance(t=6.33,P<0.05);The difference number of TAMsamong the CINII and CINIII tissue has statistical significance(t=11.12,P<0.05);The difference number of TAMs among the CINI and CINIII tissue has statistical significance(t=13.87,P<0.05).5.Thenumber of TAMsin different cervical tissues:The infiltration of TAMs is 28.84±4.84/HP,8.15±5.114/HP,3.51±1.58/HP in SCC,CIN,normal cervical tissues;The difference infiltration of TAMsamongthe SCC and CIN tissue has statistical significance(t=16.89,P<0.05);The difference infiltration of TAMs the CIN and normal cervical tissue has statistical significance(t=7.98,P<0.05);The difference infiltration of TAMs the SCC and normal cervical tissue has statistical significance(t=22.17,P<0.05).6.The relationship between the TAMs infiltrating number and the clinical and pathological features of SCC:The infiltration number is closely connected with patients'clinical stages,pathological grading and lymphatic metastasis transferand;The TAMs infiltration amount in cases of clinical stage II,lymph node metastasis and invasion depth more than 1/2 cervical wall was significantly more than the cases of clinical stage I,no lymph node metastasisthere and invasion depth less than 1/2 cervical wall,Differences are statistically significant(P<0.05);however,the TAMs infiltration amount isn't connected withpatients age,tumor size and degree of tumor differentiation.7.The relationship between the number of TAMs and the expression MMP-9 in SCC:Through relational Spearman test and analysis,there is dependency between the infiltration of TAMs and the expression MMP-9 in SCC,(r=0.250,P<0.05);with statistical significance.Conclusion:1.The number of TAMS and the expression of MMP-9 all increased gradually with the order of normal cervix tissue,CINI,CIN II,CINIII and SCC,it is suggested that they may be involved in the occurrence and development of SCC.2.TAMs and MMP-9 are also associated with tumor stage,depth of invasion and lymph node metastasis in the cervical squamous cell carcinoma,it is suggesting that they may be related to invasion and metastasis of SCC.3.The number of TAMs is positively correlated with the positive expression of MMP-9 in cervical squamous cell carcinoma,suggesting that they may play a synergistic role in the occurrence and development of cervical squamous cell carcinoma.