| Objective: To investigate the significance of human telomerase reverse transcriptase (hTERT) in the diagnosis and recurrence screening of bladder carcinoma.Methods: Immunohistochemical techniques assay were used to determine the presence of telomerase activity in voided urine samples from 42 patients with bladder carcinoma, 20 benign cases with hematuria and 20 healthy volunteers .The pathological grade of bladder carcinoma : G3:17, G2:15, G1 :10. The clinical stage of bladder carcinoma: superficial cases:25, invasive cases: 17;The number of tumors:smgle-tumor:19,multipal-tumor: 23;The time of occurrence: primary: 26,reoccurrence: 16. Immunostain was recorded as 0-3 +according to stain intensity, distribution in cytoplasm. The correlations between the expression results and tumor pathological grades, clinical stages, number of lesions, recurrence were also studied.Result: The expression of hTERT was observed in cytoplasm . Positive expression of hTERT was detected in urine samples of 36/42(85.7%) patients with bladder cancer, while no expression of hTERT in the hematuria samples and normal heal thy volunteer samples. Respectively, the positive rates of hTERT in grade G1,G2,G3 were 7/10(70%), 12/15(80%), 17/17C 100%);in superficial group and invasive group were 20/25 (80%) and 16/17 (94%);in primary group and recurrence group were 23/26(88.5% )and 13/16(81.3%);in single-tumor group and multiple-tumor group were 15/19 (78.9%) and 21/23 (91.3%) .The expression levelof hTERT in urine samples of bladder carcinoma was significantly different from that of non-bladder carcinoma (P<0.01). But, it has no correlations with cancer grade , clinical stages â– > numbers of lesion s recurrence or primaryoccurrence(P>0.05).Conclusion: The urine samples of bladder carcinoma patients have significant positive expression of hTERT and it would be a sensitively diagnostic, screening and following-up marker.
|
PDF Full Text Request