Significances Of Caveolin-1 Gene Expression And Mutation In Gastric Carcinomas

Objective: To investigate the expression of Caveolin-1 gene in normal grastric tissues, gastric atypical hyperplasias and gastric carcinomas and examine the mutation of Caveolin-1 gene in gastric carcinomas. To investigate the relationship between expression and mutation of Caveolin-1 gene and analyze the possible reason of the gene reduced expression.Methods: To detect Caveolin-1 gene expression in normal grastric tissues, gastric atypical hyperplasias and gastric carcinomas by immunohistochemical SP method, in situ hybridization and tissue microarrays technique. Identified the point mutation of caveolin-1 in gastric carcinomas by PCR-RFLP and sequence analysis.Result: 1. The positive expression rates of caveolin-1 gene in normal gastric tissues, gastric atypical hyperplasias and gastric carcinomas were gradually decreased with significant difference (P<0.001).2. The expressions of caveolin-1 gene were correlated to the malignant progression of gastric carcinoma, lymph nodes metastasis and vessel invassion, but were not correlated with patient's age and gender.3. In addition, the expression of caveolin-1 gene mRNA was positively related to that of protein (r=0.957, P<0.05).4. A point mutation of C-A in caveolin-lgene was identified at codon 132(P132L) in gastric carcinomas of paraffin embedded tissues which cases were caveolin-1 gene negatively expression with the occurrence of 18.2%(2/11) by PCR-RFLP andsequence analysis.Conclusions: 1. The decreased expression of caveolin-1 gene was one of important factors in the gastric carcinomas. Caveolin-1 gene may be a useful biomarker for the evaluation of prognosis of gastric carcinomas.2. A point mutation of C-A in caveolin-1 gene was identified at codon 132(P132L) with the occurrence of 18.2%(2/11) in caveolin-1 negative expression cases, which was approximate with that of 16.4% of Japenese scholar in literature.