Study On The Relationship Between Neurotrophic Factor-3 And Vascular Dementia

Along with the global population aging,the rate of dementiaalso raises year by year. Alzheimer disease(AD) is common at Europeand the United Stateses.Because of the high rate of thecerebrovascular disease(CVD),vascular dementia (VD)increasesgradually in Asia including our country . It not only injures thesufferer's health seriously, affects the sufferer's life quantity,but also brings heavy burden for family and societies.CVD induced obstacle syndrome of intelligence andcognition,which is called VD.VD has the followingcharacteristics:Rising urgently, course of illness is the stairstype ,many patients have the hypertension or stoke in the history.The performance is damaged for the spirit activity, the languagememory and other cognition function decrease et al. Which is alsobetter progn in the dementia, the treatment path is extensive, wecan prevent chronic disease from some extent, so if we treatmentit in early days, the attack rate will be releaser, the death ratewill be lower. When lacking blood ,neurotransmitter isless ,ultrastructure of neuron the happen change, All which inducescell dead or hurg, the quantity and quality of the contaminatedneuron is less,then affects the study and memory function of theanimal. The factor maintaining cell survival, increasingneurotransmitter synthesize, keeping from neuron apoptosisbecomes the key of prevention and cure for dementia.Neurotrophic factor-3 (NT-3) was discovered by the Errforsin 1990,It mainly exists hippocamp , pallium , cerebellum ,brainstem et al.It is a chemistry factor excred by the targetcell,which can support neuron existence and maintain thefunction of neuron .This experiment for the aged Wistar rat carryed on the methodof reformed Pulsinelli-Brierley4 blood vessel occlusioning to makeanimal model of Vascular dementia ,passed the dyeing of HE toobserved the appearance of organization , examine the diversifyof NT-3 in different tissue and different time afterischemia-reperfusion by method of immunohistochemical staining.then inquiry into the relation between NT-3 and VD .We incomed theresults as following:1. The determination for memory:Though swimming the water maze ,we discoved the number of timsfor going into the blind mistake increaser in 5 minutes and thetime for swimming the whole distance is longer .2. yeing of HE:antithetical group: cell arranged tidy, boundary was clear,theendochylema was dyed palered,nucelus presented blue,outline ofnucelus is clear, we cannot see ischemic expression.ischemia-reperfusion group: the cell appeared the dropsy or deadin different degree.3. the expression of the NT-3 in central nervous system of rat:antithetical group:we can see the expressions of NT-3 in thehippocampus, cerebellum , frontal lobe and cerebral ganglion,theendochylema was dyed the brown, a few of nucleus was dyed also, thecell presented the park, oval or triangle.At the hippocamp, the expression of NT-3 was lower on reperfusion4 hour,and depressed the nadir on reperfusion 24 hour, thenstarted to raise up and keep on reperfusion 12 day.At the cerebellum, the expression of NT-3 depressed and was thelowest point on reperfusion 4 hour,then started to raise and keep onreperfusion 12 day.At the frontal lobe , the expression of NT-3 appearanced was loweron reperfusion 24 hour,and depressed the nadir on reperfusion 4day, then started to raise up and returned to the foundation onreperfusion 12 days.At the cerebral ganglion , the expression of NT-3 appeared loweronly at 24 hour and on the foundation level on the other time.In the nonage of the reperfusion,the expression of NT-3reduced ,it hinted that NT-3 is relationed with harmful nervousafter ischemia infused. Those the conjunctive reaction theexciting toxicity, inflammation ,Apoptosis stimulated cellto respond ,which induced expressions of the NT-3 increased inthe anaphase of the reperfusion.The prevented and cure function of NT-3 for VD mainly passes thefollowing pathes: (1)Antagonizing toxicity of amino acids,stabilizing the density of Ca2+ inside the cell (2) strengtheningthe activity of enzyme for anti-oxidize, easing harmless of freeradicals (3) enhancing the activity of protein kinase C .Considered here, protection factors is advantage in the anaphase ofthe reperfusion.we drawed the conclusion from this experiment:(1)The aged rat is aphronesia through whole cerebral ischemia,which can create mode of demented animal;(2) Under the normalcondition, there is the protection mechanism of NT-3 in thebrain.The the hippocamp is sensitive to ischemia-reperfusion;the protection function of the NT-3 appear later;There exist fastand long NT-3 protection for ischemia-reperfusion at thecerebellum;The protection function of the NT-3 is more slower atthe frontal lobe;The effection of ischemia-reperfusion issmaller at the cerebral ganglion;(3) NT-3 participates thebio-chemical mechanism of VD, NT-3 can lower the occurrence forVD and defer course of illness.