The Colony Forming Ability Of CFU-GM, CFU-E And Tolemerase Activity Of Psoriatic Haematopoietic Cells

Objective: To study the colony forming ability of psoriatic hematopoietic cells and the telomerase activity of psoriatic PBMC and BMMNC, in order to probe into the in vitro proliferative activity of bone marrow-derived hematopoietic cells in psoriasis.Methods: The erythroid and granulocyte-macrophage colony forming units (CFU-E, CFU-GM) of bone marrow cells from psoriasis and healthy individuals were examined in vitro using methyleellulose culture system, the tolemerase activity of PBMC and BMMNC from psoriasis and BMMNC from healthy individuals were detected using the PCR-based telomeric repeat amplification protocol (TRAP).Results: CFU-GM of bone marrow cells were significantly lower in psoriasis groups than in healthy groups(P﹤0.05), but there was no obvious difference of CFU-E between the two groups(P﹥0.05); there was also no obvious difference of the tolemerase activity between psoriatic PBMC and BMMNC as well as psoriatic and healthy BMMNC(P﹥0.05).Conclusion: The colony forming ability of CFU-GM from psoriatic bone marrow cells is depressed, so we can infer that the proliferative activity of bone marrow hematopoietic cells in psoriasis is likely to be abnormal, and it seemd to be independent of the tolemerase.