Study Of Endotoxin Removal In Ascites Of Cirrhosis By CA/PEI Affinity Membrane

Background:At present, there is still no operative method for the treatment of refractory ascites of liver cirrhosis. Direct or concentrated reinfusion of self-ascites as well as ultrafiltration ascites reinfusion by definite objective bapertur dialyzer can not only preclud the ascites quickly but also get the albumin in the ascites back into the body. However the noxious substance such as endotoxin and bilirubin will convey back the circulation at the same time. The microfiltration membrane adsorber which can selectively remove endotoxin by employing polyethyleneimine (PEI)as affinity ligand, was prepared by blending cellulose diacetate(CA) and PEI. CA/PEI affinity memberane has achieved efficient adsorptioin of endotoxin and bilirubin from some goal solutes. We hope this membrane can meet the demands in the application of ascites reinfusion treatment .Objective:This study mainly deal with the removal of endotoxin in the ascites of liver cirrhosis by CA/PEI memberane. In order to get the appropriate condition for the adsorption of endotoxin, the ascites flow rate, the temperature and the concentration of protein were inspected and analyzed. We expected to provide a new material in the ascites reinfusion treatment which can effectively get rid of endotoxin before the ascites being concentrated or ultrafiltrated and then going back into the patients.Methods: Through static and dynamic adsorption using CA/PEI memberane, we investigated and compared the effects on deionized water and the ascites endotoxin removal in different conditions(flow rate, protein concentration and temperature). we also simultaneously paid attention to the removal of bilirubin in the ascites. Kinetic-Turbidimetric methods was...