The Immunophenotypes Of Inner Ear Hair Cell From Fetal Rat Central Nervous System In Vitro

Objective Division,culture and identification of neural stem cells form the fetal mouse to creat bases for the experimental study on treating sensorinerual deafness by transplantation of neural stem cells.Method The whole brain get from the embryonic day 14.5 fetal rat brain tissues were disassociated and trypsinizated in the D-Hank's solution. Cells were cultuled in the media composed of 20% B27, 20ng/ml EGF, 20ng/ml bFGF and DMEM/F12. Neural stem cells were identified by Nestin immunohistochemistry. Then the cells were serial subcultivated.Results Clone like cluster of neural stem cells were obtained from the whole brain. The lable efficiency of cells with the immunohistochemistry staining of Nestin was 89.86%±1.88%.Conclusions the cells from brains of fetal rat have the potential of proliferation. They can be used as donor cells to experimental research on treating neurosensory deafness with transplantation of neural stem cells.