| Objectives To explore the role of IgAl in pathogenesis of Henoch-Schonlein Purpura (HSP), we investigated the serum IgAl levels, IgAl deposition in cutaneous vessels, and C-reactive protein, et al. in children with HSP.Material and Methods ①Forty children with HSP , admitted in the first Affiliated Hospital of Anhui Medical University from 2004 sep to 2005 oct, were enrolled in the study. The diagnosis were established in the light of diagnostic criteria of HSP formulated by America rheumatology institution in 1990. None of patients had received glucocorticoids and immunosupressants in one month before hospitalization. ②HSP children were classified into three groups according to clinical manifestation: 16 cases with simplicity purpura, 14 cases with nephritis, 10 cases with gastrointestinal bleeding and ten healthy children enrolled in this study as control group. ③Serum samples were obtained from peripheral vein of all subjects in the morning after overnight fasting. Serum IgAl levels was measured by using enzyme linked immunosorbent assay (ELISA); Gather of skin specimen from purpuric skin lesion. After the samples was fixed, the pathologic alterations of tissues were observed by hematoxylin and eosin stain (HE stain) and the deposit of IgAl was observed by immunohistochemical staining, and the number of IgAl positive blood vessels were determined by image analysis system. The results were determined through the positive distribution examination and were described by mean ± standard deviation. The significant difference level is a=0.05. All data were managed with SPSS 11.5 for window. Least-significant difference and Dunnett-t of ANOVA and Pearson correlativity analysis were used.
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