Expression Of ESM-1, VEGF, CD44 In Malignant Lymphoma And The Relationship With Clinical Pathological Features

Objective TO investigate the expression of ESM-1, VEGF, CD44 in malignant lymphoma and their relationship with clinical pathological features. Methods Part 1: Immunohistochemistry detection (S-P method) and in situ hybridization method were carried out to detect the expression of ESM-1 protein and mRNA in 58 cases of malignant lymphoma respectively; Part 2: Immunohistochemistry detection were carried out to detect the expression of VEGF and CD44 in 41 cases of non-Hodgkin lymphoma. Results Part 1:Generally, ESM-1 staining was negative in the normal lymph node tissues. However, in the germinal center , we may find the expression of ESM-1 protein. The ESM-1 staining was only observed in the cytoplasm. It was negatively or weakly expressed in vascular endothelial. The intensity of staining. The intensity of ESM-1 protein staining is higher than ESM-1 mRNA. The expressin of ESM-1 protein was associated with lymphoma immunophenotyping and was negatively correlated with the clinical stage, but not with age, sex, B symptom. The expression was just correlated with immunophenotyping. Part 2: Generally, CD44 and VEGF staining was negative in the normal lymph node tissues.but some mature lymphocytes in the mantle zone and between the follicles was positively stained, the positive expression rate of VEGF and CD44 were respectively 65. 9%, 56. 1%. The expression of VEGF was associated with LDH level,but not with age, sex, stage, B symptom and immunophenotyping. while the expression of CD44 hadn' t significant correlation with LDH. Conclusions 1.The expression of ESM-1 mRNA and protein increased significantly in the malignant lymphoma.While...