Antimicrobial Activity Of Azelaic Acid And Its Effects On Melanocytes And Melanoma Cells

Objective To explore the antimicrobial activity of azelaic acid and its potential mechanism of activity; to abserve the different cultural media (nutrients and pH) contribute to antimicrobial activity of azelaic acid and find out which factor appears to be more important. To study the depigmented effect of azelaic acid, the morphological changes of melanocytes and melanoma cells influenced by azelaic acid and to investigate the effect of azelaic acid induced apoptosis of melanoma cells .MethodsThe survival rate of bacteria and the minimal inhibitory concentration and the minimal bactericidal concentration of azelaic acid were detected in Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus; the influence of various pH and nutrients on bacteria growth were compared. The inhibitory effect of azelaic acid on macromolecules biosynthesis was detected by ³H-TdR, ³H-UdR and ³H-PRO incorporating DNA, RNA, and protein respectively.The influence of azelaic acid on melanin synthesis and tyrosinase activity of human melanocytes and murine B₁₆ melanoma cells were studied; the morphological changes of human epidermal cells and murine B₁₆ melanoma cells interfered with azelaic acid were observed by TRP-1 staining.The inhibitory effect of azelaic acid on B₁₆ cells growth was measured by MTT reduction assay; the apoptosis rates were analyzed by flow cytometry; the apopotosis...