Expression Of The Nuclear Factor-kappa B(NF-κB) In The Nural Cell Of Epilepsy Rat And The Influence Of Immunoglobulin On It

Expression of the nuclear factor-kappa B(NF-κB) in the nural cell of epilepsy rat and the influence of immunoglobulin on itObjective: Discuss whether epilepsy could make the expression of NF-k B in the nural cell of rats increase, research whether the immunoglobin(IVIG) have adjust to the expression of NF-κB, so that we could use in clinic.Method: To establish the control group of epilepsy rats by being inject intraperitoneally (ip) pentetrazol (PTZ). Then the rats were divided into three groups: control group, epilepsy group (PTZ-NS), epilepsy-drug group (PTZ-IVIG). When the 2nd week of experiments , took the brain make the frozen brain tissue slice. Then technique of immunohistochemistry was used to observe the nural cell and ananlyze the immunoreactivity of the hippocampus of the rats.Conclusion: 1. With the increasing of injecting ptz , the PTZ-NS group had tendency which epilepsy incubation period was shorten and the duration was lengthen. The PTZ-IVIG group which as the increasing of injecting IVIG, it had tendency that the epilepsy incubation period was lengthen and the duration was shorten .among them , a rat had never convulted on the 13th day of experiment. It had obvious difference between PTZ-NS and PTZ-IVIG group in the incubation period and graduation of convulsion from the 5th day of experiment, 2. The expression of NF-κB of the epilepsy group was increased obviously than the control group(p<0.001). PTZ-IVIG group's expression of NF-κB was decreased obviously than the PTZ-NS group(p<0.001).Result: 1 Immunoglobulin has effect on anti-convulsion2 epilepsy can make the expression of NF-κB increase in nural...