| ObectiveTo investigate the expression of transforming growth factor β1 (TGF-β1), intercellular adhesion molecule-1(ICAM-1) and collagen type I (COL- I) in the lens epithelium cells(LECs) of streptozotocin(STZ)-diabetic rats, and the effects of TGF-β1 and TGF-β1 antibody on the expression of ICAM-1 in the culture human lens epithelium cells, and to evaluate the role of TGF-β1, ICAM-1 and COL-I in the pathogenesis of ACO,PCO and diabetic cataract. Methods120 SD rats were randomly divided into two groups: normal control and cataract. Diabetic cataract was induced by STZ intraperitoneal injection(70mg/kg in 0.1 mol/L sodium citrate buffer). Lens were examined using a slitlamp once a week. At the end of 2 weeks, 4 weeks and 8 weeks after STZ injection,eyeballs were isolated.Human lens epitheium cells were isolatedand cultured in vitro. Immunohistochemical streptavidin-peroxida.ses(S-P) method to detect the expression of TGF-β1, ICAM-1 and COL-I in normal and diabetic LECs. RT-PCR method to detect the effects of TGF-β1 and TGF-β1 antibody on the expression of intercellular adhesion molecule-1 in the culture human lens epithelium cells. Results1 .The lens of control group were always completely transparent throughout the period of experiment, and the lens of cataract group began to exhibited vacuolar stage by week 2 and floccule by week 4 ,and nearly opacity by week 8.2. The expression of TGF-β1,ICAM-1 and COL- I were significantly different between normal and diabelic LECs(P<0.01). During the progression of cataract, the...
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