The Changes Of Trace Element And Its Relation With Acute Lung Injury After Severe Trauma

BackgroundSevere trauma injures the local tissues and initiates the system patho-physiological reaction, which can lead to shock, SIRS and MODS, and even the death. MODS is the most severe complication after trauma. Lung is the most common or the first organ to be injured in the progression of post-injury MODS. And ARDS that developed from ALI is a main component of postinjury MODS. Due to the source of inflammatory cytokine production and systemic release, lung plays a main role in the postinjury MODS.Some preview researches had shown that the trace element decreased greatly and redistributed after severe trauma. Supplying TE can reduce the incidence of MOF and infection after trauma, and we propose that IB plays a great role in the postinjury MODS. Up to now, the extensive study of ALI/ARDS is mostly about the inflammatory cytokine or another media. And the relationship between TE and ALI/ARDS post-injury remains unclear.The purpose of our studies is to establish the rabbit model of severe trauma, and explore the relationship between TE and ALI/ARDS postinjury, and provide some foundation theory of clinical therapy and forensic analysis of death cause and mechanism after trauma.Materials and methodsSevere traumatic rabbit model with ISS=27 was established, and rabbits were assigned at random to three groups: 5 were in normal control group, 37 were in traumatic group, and 28 were in supplementary group. 12 dead rabbits in traumatic group were assigned to dead group, and 3 rabbits in supplementary group died after 24 hours post-trauma. The rabbits in supplementary group were given 100ml 10%glucose+0.05ml TE iv gtt at 6 hours post trauma, and then from Id to 5d after trauma, peritoneal injection were performed with 0.025ml+TE 5ml 10%GS/kg Bid. And traumatic group rabbits were just given the same dose of 10%glucose. We collected serum before trauma and 6 h, 24h, 3d, 6d, 9d, 14d after trauma respectively. Each 5 rabbits in 1d, 3d, 6d, 9d, 14d after trauma were executed group by group. Then we detected the levels of trace elements (Zn, Se, Cu, Mn), MDA, Cu/Zn-SOD, T-SOD, GSH-Px in serum and lung tissue, and the level of ICAM-1 in serum, and observed the histomorphology changes of lung, brain, heart, liver, kidney and intestine tissues.Results...