Study Of Relation Between Changes Of NF-kB, IL-6/10 And MODS In Severe Trauma: The Effect Of Trace Element

Background and ObjectiveMODS is a fatal complication after trauma ,Its high incidence is still a puzzle to medical experts. Many hypothesises were proposed on MODS. However, the fundamental causation of MODS is unbalance between proinflammatory response and anti-inflammatory response. Trace element(TE) plays an important role in the procedure of inflammatory response. Presently, it is not clear that the effect of acute TE deficiency on MODS after trauma .In our studies, to investigate the internal relation amony TE deficiency , inflammatory response and post-trauma MODS is significant for guiding the clincal therapy of trauma, for the analysis of cause of death.Materials and MethodsSevere traumatic rabbits model with ISS=27 was established, and rabbits were randomly devided into two groups, one was traumatic group with 37 rabbits and another one was supplementary group with 28 rabbits. 12 dead rabbits in traumatic group were classified as dead group, and 3 rabbits in supplementary group died after 24 hours post-trauma. All rabbits were phlebotomized from ear-edge vein before trauma and 6h, 1d, 3d, 6d, 9d, 14d after trauma during 9:00-10:00 AM . Rabbits in traumatic group were given 10% glucose (50ml/kg) ,and those in supplimentory group were done 10% glucose(50ml/kg) + 0.1ml TE by venous injection at 6 hours after trauma. During the first 5 days after trauma, Rabbits in traumatic group were given 10%GS 5ml/kg Bid,and those in supplimentory group were given 10%GS(5ml/kg)+0.025ml TE Bid. the zinc(Se),copper(Cu),selenium(Se),manganese(Mn) in serum and spleen,interleukin-6/10 in serum, blood routine and the NF-kB activity were detected. The rabbits were excuted group by group at the first, third,sixth,ninth and the fourteenth day after trauma.ResultsThe rabbits refused to eat and drink during the first 24 hours post trauma, and the weight gradually decreased in the first week after trauma. Movement of injuried limbs was seriously limited. Firstly, the body temperature dropped quickly, followed by ascending gradually.At the same time, the ear-edge vessels contracted followed by dilation. During 14 days after trauma,all of rabits were with a acceleration of respiration and heart rates.There were 12 rabbits dead with...