Studies On Quality Evalution Of Chrysanthemum Morifolium Ramat. And Preparation Technology Of Its Extracts

For the purpose of systematic quality evaluation of Chrysanthemum morifolium Ramat. and its extraction method, the following studies were carried out.1. the standard substance of 3,5-O-dicoffeoylqunic acid,luteolin-7-O-β-D-glucoside, luteolin, acacetin-7-O-β-D-glucoside were isolated from Chrysanthemum morifolium Ramat.and the structure were elucidated by UV, IR, MS, ¹H-NMR and ¹³C-NMR. The purity of 4 standards substance were over 98% by HPLC.2.according to literature's recordation, Mophology of different sources of Chrysanthemum morifolium Ramat.were carried out.3. Reversed-phase high performance liquid chromatography(Rp-HPLC) method wasused for the assay of 2 caffeoylqunic acids compounds (chlorogenic acid,3,5-O-discaffeoylquinc acid) and 4 flavonoids (luteolin-7-O- β -D-glucoside, apigenin-7-O- β-D-glucoside, luteolin and acacetin-7-O- β-D-glucoside). The gradient solventsystem(acetonitrile-water-acetic acid) and ODS reversed-phase column of Hypersil C18(4.6 mm×250mm, 5μm) were used in the quantitative analysis of the 6 components.The eluate was monitored with UV absorption at 348nm. The linear ranges of chlorogenicacid, luteolin-7-O- β-D-glucoside, 3 , 5-O-discaffeoylquinc acid, apigenin-7-O-β-D-glucoside, luteolin and tilianin were in 0.006～0.117 mg·mL^-1, 0.002～0.046mg·mL^-1,0.052～1.030 mg·mL^-1,0.010～0.202 mg·mL^-1,0.002～0.050 mg·mL^-1 and 0.003～0.052mg·mL^-1 (r≥0.9995) respectively. The recoveries were in 97.2～100. 7%, and the RSD wereless than 2.5%. The methods are simple, rapid and with good reproducibility and provide aquantitative basis for the quality assessment for Chrysanthemum morifolium Ramat. Theresults indicated that the contents of caffeoylqunic acids and flavonoids were differentbetween the species of different sources, which means that the quality of crude drugs was...