The Study Of Proteome In Spinal Cord Following Partial Dorsal Root Rhizotomy In Rats

Objective: To investigate the mechanism of plasticity after spinal cord injury, two-dimensional polyacrylamide gel electrophoresis (2-DE) technique was established through grading-up experiment condition. The differential expression of proteins was compared between the normal rats and the spinal cord injuried rats.Methods: Proteins were extracted from the lumber segments of spinal cord and separated by 2-DE. 16 SD adult male rats were divided into two groups randomly. The L₁-L₄ and L₆ dorsal root ganglions (DRG) in experimental group (n=8) were removed, but L₅ was spared. The animals were allowed to survive for 14 days. The normal rats were designed as control group (n=8). 2-DE was applied to analyze the differential expression of proteins and the peptide finger print map and amino acid sequence in differential expression of proteins were compared between the normal rats and the spinal cord injuried ones.Results: More than 800 proteins points per 2-DE gel were isolated with molecular weight ranging from 10-97 KD and pI 3-10. There was significant difference in the distribution of the three proteins between the two groups: (1) Heavy chain V region (clone X41-2) of immunoglobulin which was composed of 148 amnio acid showed up regulation in the experiment group, which its pH point was 4.09, molecular weight was 9.61KD. (2) At the pH point 9.10, molecular weight 10.12KD, Chain A, CalmodulinIQ-Aa Domain Complex which was composed of 127 amnio acid showed down regulation in the experiment group. (3)Fructose-bisphosphate aldolase, which was composed of 364-amnio acid showed down regulation in the experiment group, which its pH point was...