Expression Of Sema3A,NP-1 In Spinal Cord And NP-1 In Spared Dorsal Root Ganglion After Partial Dorsal Root Rhizotomy

Objective: To explore the change in expression of nerve growth inhibitor Sema3A in spinal cord and its receptor neuropilin-l(NP-l) in spinal cord and spared dorsal root ganglion after unilateral partial dorsal root rhizotomy of adult cats. Furthermore, to offer datas for exploring roles of nerve growth inhibitor in spinal cord plasticity.Method: 15 adult cats were used. They were divided into 3 groups: normal control group, unilateral partial dorsal root rhizotomy 7^th and 14^th day groups ( i.e. rhizotomy of unilateral L₁-L₅, L₇-S₂ dorsal roots, leaving L₆ dorsal root as a spared root . The animals were allowed to survive 7 days and 14 days). The animals were purfused and fixed with 4% paraformaldehyde .Their L₃, L₅, L₆ segment of spinal cord were made into 35 μm thickness frozen sections and L₆ dorsal root ganglia (DRG) in operated side were made into 20 μm thickness sections. Sections of spinal cord were stained with specific Sema3A (1:1600) and NP-1 (1:500) antibody by immunohistochemistry ABC method and L₆ DRG were stained with NP-1 antibody (1:600). Mean optical density (OD) of Sema3A and NP-1 immunoreactivity in superficial laminae of the dorsal horn was measured with image analysis system. The number of...