Apoptosis Induced By Flavone Of Yunnan Purpleblow Maple On Lung Cancer Cell Line YTMLC In Vitro

Objective: This study was designed to primarily investigate the inhibitory effects and the mechanisms of flavone of Yunnan purpleblow maple-inducing apoptosis in lung cancer cell line YTMLC in order to acquire more information about flavone'functionsMethods: MTT (Methy thiazolyl tetrazolium) assay was used to evaluate the inhitory effects of flavone of Yunnan purpleblow maple in different concentration and time. Alteration of morphology of flavone induced apoptosis on YTMLC cell line in vitro were observed by invert microscope, fluorescence microscope, sanning electron microscope and transmission electron microscope. FCM(the fluorescence flow cytomtry) DNA assay and TUNEL(Terminal deoxynuxleotidel transferase mediated uridine nucleotide and labeling) were applied to detect the changes of apoptotic rate at early and late apoptosis process and cell proliferation and alteration of cell cycle phase. DNA fragmentation (DNA ladder) assay indicate that flavone induced the apoptosis on YTMLC cell line.Resμlts: Flavone of Yunnan purpleblow maple could inhibit the growth of YTMLC and the inhibitory effects showed concentration-time-effect pattern. Apoptotic morphology obvious changes were observed by invert microscope, fluorescence microscope, sanning electron microscope and transmission electron microscope. FCM assay indicated that most of the cells were arrested in S phase and the apoptotic peak appeared. During the prolong of incubation time, the apoptosis rate was increased. The results of TUNEL and DNA ladder certified that there are apoptosis.