The Regulation Role Of Peroxisome Proliferator-Activated Receptor-Gamma In The Growth Of Digest Tumors

Objective: To investigate the regulation role of PPARγ in the growth in human digest tumors in vitro, and to examine the interrelation between expression of PPARγ or RXRα and sensitivity of its ligand , and to discuss the effects of these ligands on the expression of PPARγ and RXRα , cell cycle and cell apoptosis. Further to clarify the action and the possible mechanisms of PPARγ activation on the chemoprevention of digest tumors.Methods: 1.The expression of PPARγ and RXRα in the presence of PGZ (ligands of PPARγ) or 9-cis-RA (ligand of RXRα) or both were examined by RT-PCR. 2. Antiproliferative effect in the MGC803, SMMC7721 and Lovo cell lines treated with PGZ or 9-cis-RA or both were evaluated by cell viability using MTT assay. 3. MGC803 cell line treated with PGZ or 9-cis-RA or both was used to study the effects of PPARy activation on cell apoptosis and cell cycle by flow cytometry. The expression of CDK and CyclinDl in MGC803 cells was examined by RT-PCR. 4.The effects of PPARy activation on cell apoptosis was studied by Hoechst33342/PI staining and DNA agarose gel electophoresis in MGC803 cell line treated with...