Effects On The Several Malignant Phenotypes Of HPTTG Gene Transfection In A549 Cell

Background:Chromosomal number abnormality is one of the characteristic changes in tumor cells. The mechanism of sister chromosome separation is not very clear though the phenomenon has been discovered for a long time. The investigation of mechanism made a lot of advancements in the recent years. We presumed that the mechanism of sister chromosome separation is controlled mainly by securin/PTTG and separase/ESP1, and securin can inhibit the activation of separase. In the mitosis anaphase, APC(anaphase-promoting complex) degradates securin, activates separase, and hydrolyzes cohesin/SCC1. Then sister chromosome separates on the spot of kinetochore, moves two-pale, and distributes two daughter cells.We have already discovered that up-regualiation of separase can reduce the charomosomal number, inhibit the cell proliferation and induce apoptosis in tumor cells. Owing to securin which PTTG(pituitary tumor-transforming gene)codes is one of the important modulin participating in the process of sister chromatid segregation. We want to know the effects on chromosome number, cell cycle,proliferation and apoptosis when we up-regulate the expression of PTTG in tumor cells. The investigation of above-mentioned topic will help us understand the disturbance of chromosome segregation and the relationship between several malignant phenotypes in tumor cells, and provide the new viewpoint in gene therapy for tumor cells.Objective:Observe the changes of hPTTG up-regulation on chromosome number,cell cycle, cell proliferation and apoptosis in A549 cells, and explore its possible regulatory mechanisms of promoting proliferation further. Methods:(1) Cloned the DNA sequence encoding hPTTG gene.(2) Constructed the eukaryotic...