Expression Of Osteopontin And CD44v6 MRNA And Its Relationship With Invasion And Metastasis In Ovarian Cancer

Objective: Ovarian cancer is the first case fatality ratio in gynecological malignancies, and its incidence shows a upgrade tendency. The total 5 years survival rate is about 45% and there is a significant prognosis between difference grades. The main reasons leading to death are the metastasis and recurrence of tumors. Three quarters of patients present with International Federation of Gynecology and Obstetrics stage III-IV disease, chiefly because there is no reliable screening technique for invasion and metastasis. So markers with a well-defined and validated role in monitoring of cancer metastasis are important. By this way, we can reduce the death rate and improve the living quality. Recently, many studies have shown that, osteopontin(OPN) can interact with CD44v6,and expressed in numerous human cancers. OPN activates CD44v6 mediated pathways to promote cell survival, chemotaxis, homing and adhesion, each potentially enhancing metastatic behavior in tumors. To investigate the correlation between osteopontin and CD44v6 mRNA with clinicopathologic features of ovarian tumor ,we used semi-quantitive RT-PCR to detect the mRNA expression of OPN and CD44v6 and elucidate their role in tumor invasion and distant metastasis.Methods: The expression of OPN and CD44v6 mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR). The relationship between the relative content of OPN and CD44v6 mRNA and clinicopathologic features of ovarian cancer was analyzed.Results: The was statistically correlated with histology classification of OC(P<0.05).The OPN and CD44v6 expression in advanced(stage III-IV) OC were higher than that of initial stage(I-II) (P<0.05),as well as the...