Expression Of GST-π And MRP In Human Skin Carcinoma Tissue And Their Correlation

Background and objectiveBoth basal cell carcinoma (BBC) and squamous cell carcinoma (SCC) are the commonly malignant tumors of skin and their carcinogenesis is a complex process involving many factors, which has not been well understood so far. Recently years the concern has been focused on the mechanism of the multiply-resistant drugs. It has been found that GST-Ï€ has the ability to catalyze the GS-X complexes which comprise of deoxidized GSH and the non-ionised medicine, and then transport it outside the cell to deoxidize it, which results in both tissue's and cells' higher resistance to the carcinogens and anti-drugs. Multiply-resistant protein (MRP) is capable to deoxidize the toxin and offset the resistant response by transporting GS-X complex and involving in cytoplasm vesicle transportation, which change the drug concentration inside the cells. However, the expression of the MRP and GST-Ï€ and their roles in the BCC and SCC tissue are not clarified. In this study, we adopted in situ hybridization and immunochemistry to investigate the expressions of GST-Ï€ mRNA and protein and that of MRP protein in BBC and SCC tissue and in the normal skin tissues so as to investigate their roles and correlation in skin carcinoma and the significance. Materials and methods:The tissues of basal cell carcinoma and squamous cell carcinoma were obtained from the patient underwent plastic surgery in the First Affiliated Hospital of Zhengzhou University, etc. The tissue was collected from the face, neck and extremes. The normal skin tissue was collected from face and necks were acquired in the...