| Objective The purpose of this study is to investigate the preventive and therapeutic effects of actived protein C(APC) on acute gastric mucosal lesion(AGML). Material and methods 48 rats were random divided into eight groups,namely normal control group(A1,A2),water-immersion restraint stress(WRS)group(B1,B2),rhAPC group(C1,C2), physiologic saline group(D1,D2).In the present study, we examined this possibility by using a rat model of WRS-induced gastric mucosal injury.Changes in gastric lesion index(UI),plasma levels of TNF-αand IL-6 and IL-1β,and mitochondrial transmembrane potential were measured at indicated time points after WRS.The pathologic changes were examined with optical microscope.Results The results showed that the significant increasing of TNF-αand IL-6 in plasma and UI compared to control animals(P<0.01),the significant degradation of mitochondrial transmembrane potential.The levels of TNF-αand IL-6 were significantly higher in B1 group than in B2 group(P<0.01).But the levels of IL-1βfrom 2 to 3.5h after WRS were not significantly different from those in control animals. After APC was administered intravenously 30 min prior to WRS,the levels of TNF-αand IL-6 and UI were significantly lower in C1 group than in B1 group(P<0.01).But the levels of IL-1βin animals pretreated with APC after WRS 3.5h were significantly higher vs B2 group. APC inhibited the degradation of mitochondrial transmembrane potential (P<0.01,vs.saline).Conclusion This study suggests that APC reduces stress-induced gastric mucosal injury by inhibiting the increasing of TNF-αand IL-6 in plasma and UI, by inhibiting the degradation of mitochondrial transmembrane potential and by...
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