| Objective:To observe the effect on PGE2, SS, MTL, TNF-a, EGF, TGF-a, and histological changes of acute gastric mucosal lesion rats treated by different acupoint combination groups, and to analyze the protective effect of them.Method:60 SD rats were divided randomly into 6 groups,10 rats each group. Group A was blank control group. Group B was model group. Rats in group C (combining points on the same meridians) was electroacupunctured in Zusanli and Chongyang. Rats in group D (combining points on the exteriorly-interiorly related meridians) was electroacupunctured in Zusanli and Taibai. Rats in group E (combining points on the meridians sharing the same name) was electroacupunctured in Zusanli and Hegu. Rats in group F (combining points on the unrelated meridians) was electroacupunctured in Chengjin and Jinggu. Acute gastric mucosal lesion rat model was built by ethanol ingestion. Gastric mucosal lesion index was recorded and histological changes of gastric mucosal tissues were observed under microscope. Radioimmunoassay was used to determine contents of PGE2,SS,MTL,TNF-α, and Immunohistochemistry for expression of EGF and TGF-α.Results:①Compared with blank control group, UI was increased in other 5 groups, especially in group B, D, and F (P<0.05 or P<0.01). Compared with model group, UI was decreased in group C, D, E (P<0.05), no significant decrease in group F. Compared with group F, UI was decreased in group E (P<0.05).②In group C, D, and E, gastric mucosa looked bloom, few punctuate hemorrhage, epithelium tissue was integrated, glandular organs was regular, few inflammatory cell infiltration, gastric mucosa was lightly congestive. In model group, and group F, gastric mucosa was congestive, dropsied, punctuate hemorrhage and erosion, epithelium tissue was incomplete, glandular organs was damaged and disorder, nucleocytoplasmic was fuzzy, capillary proliferation, Substantial inflammatory cell infiltration.③Compared with group A, content of PGE2 were similar in each group. Compared with group B and F, content of PGE2 increased in group C (P<0.05).Content of SS was decreased in group B, D, E, F, compared with group A (P<0.01). compared with group B, content of SS was obviously increased in group C, D, E (P<0.05 or P<0.01), and that in group C was obviously higher than group D, E, F (P<0.01)Compared with group A, content of TNF-αwas increased in group B, C, D, E, F (P<0.05 or P<0.01). Compared with group B and F, that was decreased in group C, D, E (P<0.01), and that in group C, D was obviously lower than group E (P<0.05 or P<0.01)No significant difference showed in content of MTL in each group.④EGF, TGF-αexpressed in gastric mucosa of every group, located mainly in gastric gland. Compared with other groups, EGF expression was increased obviously in group C, D (P<0.01 or P<0.05). Compared with other 5 groups, TGF-a expression was increased obviously in group C (P<0.01 or P<0.05)。Conclusion:①7 days after acute gastric mucosal lesion, UI was increased, content of GM rats SS was descended, and TNF-αof gastric mucosa and plasma was increased. There might be some relationship between abnormity of SS and TNF-αwith GM pathomechanism.②Reparation effect on GM showed in 3 kind of point combination:acupoints of same Meridians, acupoints of Exteriorly-Interiorly Related Meridians, acupoints of Meridians Sharing the Same Name. But no significant effect showed in unrelated points.③The reparation effect of acupoints of the Same Meridians might be to inhibit TNF-αrelease, promote PGE2, SS synthesis, and promote expression of EGF and TNF-αin gastric mucosa. The reparation effect of acupoints of the Exteriorly-Interiorly Related Meridians might be to regulate TNF-αand SS synthesis, promote expression of EGF. The reparation effect of acupoints of the Meridians Sharing the Same Name might be to regulate TNF-αand SS synthesis and release.
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