The Effluence On Expression Of Apoptosis,heat Shock Protein, Smac/diablo In Acute Gastric Mucosal Lesion After Pro-moxibustion Zhongwan And Zusanli Point

Objective:To observe the effluence on expression of apoptosis,heat shock protein60(HSP60),heat shock protein70(HSP70),Smac/Diablo in acute gastric mucosal lesion of rat's epithelial cells and the protection of gastric mucosa after pro-moxibustion "zhongwan" and "zusanli" point. Probe endogenous protective mechanism by moxibustion treatment of digestive diseases, and result in experiment evidence for treatment of the digestive diseases.Method:Sixty rats were randomly divided into four groups, such as blank control group, model group, moxibustion at "Zusanli" and "zhongwan" and moxibustion at control points groups. Gastric ulcer model was established by intragastric administration with absolute alcohol, before pro-moxibustion once daily and continuously for 8 days. At the end of the experiments, the assaying the content of HSP60,gastric mucosa, cell apoptosis and Smac by immunohistochemical method.Results:Absolute-alcohol model was successfully established as long as UI value in model group's was significantly higher than in blank group's. There are no statistical differences between blank group and moxibution at points group, in which UI value was significant lower than model group and moxibustion at control points group. HSP60,HSP70 values in model group,moxibustion at points group,moxibustion at control points group were significantly higher than blank group. It proved after intragastric administration with absolute alcohol, both HSP60 and HSP70 expression were enhanced in gastric mucosa. The expression of HSP60,HSP70 in moxibustion at points group of gastric mucosa decreased highly (P<0.01),compared with model group and moxibustion at control points group. The expression of apoptosis Smac/Diablo in model group was significantly increased than blank group (P<0.05), and lower in moxibustion at points group than model group and moxibustion at control points group (P<0.01). The apoptosis in moxibustion points group were inhibited stronger than in the moxibustion at control group.Conclusion:The pretreatment of moxibustion points group can relieve the damage of absolute alcohol to gastric mucosa. In order to protect gastric mucosa, moxibustion can induce HSP60,HSP70 to express excessively by suppressing Smac's expressing to suppress the expressing of apoptosis and harmful stimulation. The effect of moxibustion points group is more remarkable than of moxibustion at control points group, which proves the protection of gastric mucosa by moxibustion has a specificity of acupuncture.