Isolation Of Adenosine In Panax Ginseng And Lepidium Peruvianum And Quantitation Of Monomer Ginsenoside In Panax Ginseng

Pcmax ginseng and "Peruvian ginseng" that is Lepidium peruvianum Chacon (Maca) were investigated on chemical composition in order to find natural compounds with bioactivity. A non-saponin compound, which is adenosine, was isolated from the flower of Panax ginseng and stem and leaf of Peruvian ginseng. The isolation and purification method of adenosine was researched. The contents of ginsenoside Rg₁, Re, Rb₁, Rc, Rb₂, Rd, compound K and Rg₃ in Panax ginseng and its processing products were quantitated by Reverse Phase High Performance Liquid Chromatography (RP-HPLC); The contents of adenosine in Panax ginseng and its processing products, Peruvian ginseng were determined by RP-HPLC; the elements in stem and leaf of Peruvian ginseng were studied on quanlitative and quantitative analysis by Plasma Optical Emission Spectrogragh.There were a lot of reports on ginsenosides and their pharmacologic action from ginseng, but studies on non-ginsenosides were rather fewer and not further. Non-ginsenosides were focused on this experiment. Adenosine may be one active component for strengthening yang of flower of Panax ginseng.Peruvian ginseng has been used for an edible hyperalimentation crop in the Andes mountains area of Peru for thousands of years. The traditional medicative action, such as improvement of vigor and endurance, sexual function, was widely spread. Adenosine in root of Peruvian ginseng introduced from Peru and Peruvian ginseng introduced from Peru growing in Fusong for four months were determined by RP-HPLC in order to ascertain the effective parts, active compounds and targets of pharmacologic action of Peruvian ginseng and do the groundwork for appreciation of quality control of Peruvian ginseng. The results showed that the content of adenosine in stem and leaf in Peruvian ginseng which introduced from Peru growing in Fusong four months was highest, the root of Peruvian ginseng introduced from Peru ranked the second, and the content of root of Peruvian ginseng introduced from Peru growing in Fusong for four months was the lowest. Adenosine was isolated and identified from Peruvian ginseng for the first time.The flower of Panax ginseng and stem and leaf of Peruvian ginseng were extracted by methanol in ultrasonic wave. the concentrated extract after extraction was isolated by silica gel column chromatography and the n-butanol extractum was eluted by chloroform and methanol in the way of gradient elution. The compound was identified as adenosine by physico-chemical property, spectrum analysis (ultraviolet spectra, infrared spectra, nuclear magnetic resonance). Adenosine was found in flower of Panax ginseng for the first time. The operational approach is simple, moreover, the eluting agent can be repeatly used.