Study On The Immunotoxic Effects Of Microcystins From Cyanobacteria Bloom In Shanzi Reservoir, Fujian

Objective: Study on the specific immunity and nonspecific immunity effects of microcystins from cyanobacteria bloom in Shanzi reservoir and its mechanism in mice.Method:1. Mice spleen lymphocytes were suspended at a density of 2×10~6cells/ml in RPMI 1640 medium supplemented with 10% fetalcalf serum and were distributed in fat-bottomed 96-well microtiter plates for six well, Mice spleen lymphocytes were treated with MC-LR at the doses of 0,1,5,10μg MC-LR /ml for 72h(Our previous work had indicated the presence of microcystin in cyanobacteria bloom collected from shanzai and showed that the main toxic component of cyanobacteria bloom extract is MC-LR) and tested for lymphoproliferation; Mice peritoneal macrophages were also suspended at a density of 1×10~6cells/ml in RPMI 1640 medium supplemented with 10% fetalcalf serum and were distributed in fat-bottomed 96-well microtiter plates for six well, then peritoneal macrophages were treated with MC-LR at the doses of 0,1,5,10μg MC-LR /ml for 24,48,72h and tested for proliferation.2. 40 ICR male mice were weighted and randomly assigned to four group on body weight and was administered by i.p. for 7 days at four doses of 8, 16, 24μg MC-LR /kg body weight. On the eighth day the mice were sacrified and tested for DTH,HC50,the indexes of the clearance rate of carbon particles and SOD,GSH-PX,MDA and LDH in the spleen, thymus and spleen was investigated with H.E staining and body weights index. 3. 40 ICR male mice were weighted and randomly assigned to four group on body weight and was administered by i.p. for 7 days at four doses of 8, 16, 24μg MC-LR/kg body weight. on the eighth day the mice were sacrified...