The Quality Control Study Of Bupleurum Chinense DC.

Bupleurum chinense DC., which belongs to Umbelliferae family, is one of traditional Chinese medicines in common used with its radix. Abounding in natural resources, this species is widely distributed in the north of China.In this paper, studies on chemical constituents and quality control of the radix of Bupleurum chinense DC. are presented throughly. Seven compounds were isolated from the radix with Silica-gel culumn and preparative thin layer chromatography, etc.These compounds were identified on the basis of physicochemical properties and spectral data, which are angelicin(W-1), kaemferol (W-2), rutin (W-3), quercetin (W-4), saikosaponin a.(W-5), daucosterol (W-6), β-sitosterol (W-7), respectively.In this paper, studies on the contents of saikosaponin a and total saikosaponins of Radix Bupleuri from 9 different sources are carried out. Saikosaponin a was used as the chemical reference substance to establish RP-HPLC and visible-spectrophotometry methods for the determination of contents of saikosaponin a and total saikosaponins.HPLC-fingerprint spectrum analysis of 9 different sources of Bupleurum chinense DC. was developed.These results can be served as basement for the further study of Bupleurum chinense DC. They also afford reliable experimental basis for the application of HPLC in quality control of Traditional Chinese Medicine.