The Effect Of Sodium Ferulate On Tubular Cell Apoptosis Induced By Cisplatin In Vitro

OBJECTIVE:Cisplatin,a commonly used chemotherapeutic agent,has a major limitation because of nephrotoxicity.Recent studies have shown that cisplatin caused apoptotic cell death in renal tubule cells and in a dose- and duration-dependent manner in cultured proximal tubule (LLC-PK1) cells,as evidenced by DNA laddering and TUNEL.Furthermore,antioxidant ameliorates cisplatin-induced renal tubular cell death through of death receptor-mediated pathways.Sodium ferulate(SF),extracted from a traditional Chinese herbal medicine,has potent antitoxidant and anti-inflammatory activities.It was hypothesized that sodium ferulate,as an antioxidant,would be cytoprotective in cisplatin-induced apoptosis.This study is to observe the effect of sodium ferulate on tubule cell apoptosis induced by cisplatin in vitro and to investgest its mechanism.METHODS: Cultured normal rat kidney tubular epithelial cells (NRK-52E) were divided into control group,cisplatin-induced group and different dose of sodium ferulate group.When cells were adherent,sodium ferulate were added to cultures,which indicated,to a final concentration of 100,250,500,1000uM.