Primary Study Of Radioprotective Mechanism Of Fluticasone Propionate And Its Effect On Histopathologic Changes Of Irradiated Guinea Pigs' Nasal Mucosa

Objective (1) To observe histopathologic changes of irradiated guinea pigs' nasal mucosa treated with fluticasone propionate (FP) , (2) To detect concentration of IL-1,IL-6 and TNF-α in serum of irradiated guinea pigs after using FP with ELISA and to study FP's radioprotective mechanism.Methods 100 health guinea pigs were used and divided into 4 groups randomly: the control group(I),the administration group(II),the irradiated group(III) and the administration group after irradiation(IV), 25 guinea pigs for each group.the I and II group did not be irradiated,the III and IV group were performed irradiation by the WDVE-6MeV linear accelerator, giving 5Gy one time a week for three weeks through X-ray irradiating to establish the animal irradiation injury model.the II and IV group received fluticasone propionate nasal spray on the second day after three weeks irradiation, spraying double nasal cavities, one time every day and one spray each side. 5 animals per one group were sacrificed randomly at 1week, 1,2,3,4 month after irradiation, the histopathologic changes were observed under optical, scanning electron and transmission electron microscope. At the same time, blood were drawn from the heart and the concentration of IL-1,IL-6 and TNF-α in the serum were measured by ELISA.Results (1) The administration group(II): The abnomal change did not be observed in the guinea pigs nasal mucosa received FP without irradiation. the nasal mucosa was integrity, the submucosa glandular organ was integrity and the function was normal. The ciliary cylindrical epithelium construction could not be found any damage and ciliary exfoliation. (2) The irradiated group(III): The early...