Pharmaceutical Study Of Compound Propolis Cream

The compound propolis cream is comprised of propolis,Radix sophorae flavescentis,Radix scutellariae,Radix saposhnikoviae.It is applied mainly to treat herpes zoster and eczema. To pharmic research and develop a new preparation of the Compound propolis, which is effective on herpes zoster'sufferer. To discuss the rationality of the Compound propolis contains traditional Chinese medicine through the safety and efficiency of the new preparation.The extraction of Chinese traditional medicine has divided into two parts: extraction of propolis is used microwave and ultrasonic treatment, with the content of total flavonoids as the evaluating index , then selected the optimal extraction condition of propolis by orthogoral test. The results as follows: 80% alcohol as solvent and dose of 15-fold medicinal materials, the power of microwave 600W, the treatment time of microwave 5minutes, and the ultrasonic extracing two times, 20min in each time at 70℃in water.The extraction technology of other traditional Chinese medicine was water extracting and alcohol precipitation, namely using orthogonal test, with matrine and the whole solids as index, to study the effect of four extracting factors (the amount of water, the extracting time and times and the concentration of alcohol precipitation) by HPLC.The result of optimal extracting conditions was added 8 fold of water, extraced two times for 1hr in each time, the 60% concentration of alcohol precipitation. As a result, the process is reasonable and favorable for extration process.The preparation reasearch was mainly selected appropriate base to prepar the o/w type creams by uniform design. Namely the optimization of preparation process by emulsion solvent method, using morphological characteristics of creams, partical size distribution and the height of oil layer in the centrifuged emulsion as evaluating criterion. The optimum matrix composition and technology were the oil phase of volume faction is 27%, emulsifying agent 5%, HLB 11 and emulsifying temperature 80℃, then the emulsion prepared by this kind of composition and technology was homogeneous, exquisite.The quality standard for compound propolis cream was established the identification of Radix scutellariae and propolis by TLC, set down the range of pH, and erected the contend of matrine by HLPC and total flavonoids by spectrophotometry. The result was that the methods of identification was simple and feasible; the range of pH was 5.0～7.0; and the calibration curves of matrine were linear between 40.00-200.00 mg.L^-1 (r=0.9998), the average recoveries of matrine in compound propolis creams were 98.44%, RSD were 1.97%(n=6), the limits of matrine'contents in creams was no less than 0.7mg/g; and the linear range of rudin was 7.92μg/ml-47.52μg/ml (r =0.9 997), the...