Experimental Study On Treatment Of Endometriosis With Xiao Chaihu Tang In Rats

Objective:To investigate the effects of Xiao Chaihu Tang on endometriotic tissue and explore the probable mechanisms by means of the Endometriosis (EMs) rat models.Methods:(l)The rat EMs models established by surgical operation were divided into 4 groups.They were treated by water, Xiao Chaihu Tang,Danazol and Xiao Chaihu Tang plus Danazol for 4 weeks,respectively.(2)By observing and measuring EOS'volume before and past treatment and the height of endometrial epithelium(H.),to evaluate the therapic effect of each group.(3)Ultrastructural change in EM and EOS of each group were observed by elevtron microscopy.(4)COX-2 and P450 arom protein expression in EM and EOS of each group were observed and analyzed by immunohistochemistry method.Results:(l)The rat models of surgically induced endometriosis were established successfully.(2)Compared with the control group, the EOS'volume of the treatment groups was obvisously reduced(P<0.01) ,specially the Xiao Chaihu Tang plus Danazol group.(3)The diminution of the height in the treatment groups was more significant than the control group(P<0.01). The dimished rate of the height was obvious in Xiao Chaihu Tang plus Danazol group.(4)The glandular cell in the endometrial implant after therapy with Xiao Chaihu Tang showed characteristic feature of apoptosis(APO),which displayed the cell decreased in size, karyopyknosis, cytoplasmic and nuclear chromatin condensation,density increased and many apoptotic bodies among cell.Whereas some stromal cell displayed degeneration and necrosis. Many nectrotic cell and auto phagosome were commonly seen in the Danazol group.