Study On The Antioxidation Effect Of Water Soluble Extracts From Salvia Miltiorrhiza

Objective:To study the antioxidation effect of water soluble extracts from Salvia miltiorrhiza onthe rat model of oxidative damage byγ-irradiation, the middle-aged and elderlyperople.Methods:1. In the animal study, sixty Kunming mice were randomly divided into model group, low dose group, medium dose group, high dose group and normal control group. The model group and the normal control group were administrated total salvianolic acids at a dose of 0 mg/kg.BW/d, the three dose groups were 6.4 mg/kg.BW/d, 12.7 mg/kg.BW/d, 38.1 mg/kg.BW/d. The erythrocyte SOD activity, blood GSH-Px activity were determinated before the study and one month later. Thenγ-irradiation was given except the normal control group, 4 days later the erythrocyte SOD activity, blood GSH-Px activity, the SOD activity, GSH-Px activity and MDA content in the mice liver were measured.2. In the human study, 120 middle-aged and elderly people were randomly divided into control group and test group. The test group were given total salvianolic acids at a dose of 1.27 mg/kg.BW/d. The control group were not treated with anything. The changes of the erythrocyte SOD activity, blood GSH-Px activity and serum MDA content before the study and 3 months later were studied to observe the antioxidation effect to human body of water soluble extracts from Salvia miltiorrhiza.Results:1. In the animal study, there was no significant difference of the erythrocyte SODactivity and the blood GSH-Px activity between the five groups before the study (P> 0.05).After given the water soluble extracts of Salvia miltiorrhiza 30 days, the erythrocyte SOD activity significantly increased in the medium dose group compared with the model group and the normal control group(P<0.05). The erythrocyte SOD activity increased in the low dose group and high dose group compared with the model group and the normal control group, but there was no significant difference(P>0.05). 4 days later after givenγ-irradiation, the erythrocyte SOD activity significantly decreased in the model group compared with the normal control group(P<0.05). The erythrocyte SOD activity significantly increased in the three dose groups compared with the model group (P<0.05).After given the water soluble extracts of Salvia miltiorrhiza 30 days, the blood GSH-Px activity significantly increased in the medium dose group compared with the model group and the normal control group(P < 0.05). There was no significant difference of the blood GSH-Px activity between the low, high dose group and the model group, the normal control group(P>0.05). 4 days later after givenγ-irradiation, the blood GSH-Px activity significantly decreased in the model group compared with the normal control group(P < 0.05). The blood GSH-Px activity significantly increased in the three dose groups compared with the model group(P<0.05).4 days later after givenγ-irradiation, the liver MDA content was significantly higher in the model group compared with the normal control group(P<0.05). The liver MDA content in the low dose group was lower than the model group, but there was no significant difference (P>0.05). The liver MDA content was significant lower in the medium dose and high dose group compared with the model group(P<0.05). The liver SOD activity was significantly lower in the model group compared with the normal control group (P<0.05). The liver SOD activity was significantly higher in the three dose groups compared with the model group (P<0.05). The liver GSH-Px activity was significantly lower in the model group compared with the normal control group (P<0.05). The liver GSH-Px activity was significantly higher in the medium and high dose group compared with the model group (P<0.05). The liver GSH-Px activity was higher in the low dose group compared with the model group, but there was no significant difference(P>0.05).2. During the 3 month human study, 12 persons quit for various reasons. There was no significant difference of the erythrocyte SOD activity, the blood GSH-Px activity and the serum MDA content between the test group and the control group before intervention (P>0.05). There was no significant difference of the erythrocyte SOD activity, the blood GSH-Px and the serum MDA content in the control group before and after the intervention (P>0.05). In the test group, the serum MDA content decreased from 3.28±0.63 nmol/ml before intervention to 2.45±0.57 nmol/ml after intervention, which showed significant difference (P<0.05). The blood GSH-Px activity increased from 63.64±8.74 U/ml before intervention to 98.99±11.26 U/ml after intervention, which also showed significant difference(P<0.05). There was no significant difference of the erythrocyte SOD activity before and after intervention in the test group (P>0.05).Conclusions:1. The 12.7 mg/kg.BW/d dose of total salvianolic acids in the water soluble extracts from Salvia miltiorrhiza showed the strongest effect of antioxidatin in the animal study. It can significantly increase the erythrocyte SOD activity and the blood GSH-Px activity, it can also significantly increase the erythrocyte SOD activity, the blood GSH-Px activity, the liver SOD activity and the liver GSH-Px activity after given theγ-irradiation. It can significantly decrease the high liver MDA content induced by theγ-irradiation.2. The total salvianolic acids in the water soluble extracts from Salvia miltiorrhiza show very strong effect of antioxidation in the human study. It can significantly increase and the blood GSH-Px activity, and also significantly decrease the serum MDA content in the test group. However, it shows no effect on the the erythrocyte SOD activity.