Bone Histomorphometry Study About The Effects Of Mechanical And Biological Factors On Osteoporosis

Osteoporosis is a systemic skeletal disease characterized by low bone mass and microarchitectural deterioration of bone tissue,with a consequent increase in bone fragility and susceptibility to fracture.Osteoporosis is one of the world's most popular human diseases. With changes in the structure of the population, the aging of the world's population, the trend is becoming increasingly obvious. The corresponding aging population, the incidence of osteoporosis, and there is a significant positive correlation between. It was once regarded as the elderly, especially diseases in postmenopausal women. This shows that the elderly and the desperate lack of estrogen is a major risk factor of osteoporosis.Bone histomorphometry this paper observed from different levels of exercise on mechanics Biological factors quantitative analysis of the impact of osteoporosis. Using demineralized main sections, and micro-slice CT was used to analyze three methods. Decalcification was observed morphologic changes were adopted, and micro-slice CT for demineralized bone tissue morphology. The results of the two methods were compared.We know that change is functional changes in the morphology and function of morphological change in turn affects the structure. Combining quantitative and morphological changes also help to reveal the function of pathological lesions and nature of the relationship.Bone histomorphometry is Stereology,Biomedical histomorphometry of aspecial branch. Quantitative analysis of bone is hard tissue biopsy in the two-dimensional image, using stereological methods derived reflect bone remodeling. The three-dimensional bone structure parameters. This has in the diagnosis of metabolic bone diseases such as osteoporosis has played an important role.8-month-old rats were used experimental animals, randomly divided into : suspension,ovariectomy, drugs, the three groups. Using demineralized sections, undecalcified sections and micro CT Quantitative analysis of three methods. Model results are analyzed. Suspension : Suspended group was divided into:control group, Suspended contrast group , Suspended loading 1N,2N,3N,4N,5N. Ovariectomy is divided into 3 groups:control group,ovx contrast group,ovx loading 1N, ovx 2N ,ovx 3N, ovx 4N , ovx 5N.. Drug A group divided into: control group and drug group. Group B drugs into ovariectomy drugs, ovariectomy contrast group and control group, and only a fluorescent labeling in this group. Micro-CT rats were mainly made in the suspension of 12 weeks, 12 weeks ovariectomized, control 12-week data. In this paper,the Image-pro Plus is the image analysis software (provided by the Department of Medicine at Jilin University).According to data analysis of the results :Bone's modality of the two load group in ovx and suspend is very different from the contrast group. Bone histomorphometry parameters such as trabecular bone area, trabecular separation, trabecular number were significant differences. Note mechanical loading for osteoporosis caused the lack of estrogen and disuse has a greater positive effect.In the drug group,the drug treated group than the control group's the bone histomorphometry parameters have improved significantly, bone formation and bone structure have shown marked improvement. dynamic parameters of the drug group showed significantly lower than in the control group into bone;This shows that the lack of estrogen salmon calcitonin for the treatment ofosteoporosis in a significant role.Micro-CT results of the parameters, ovariectomized suspended for 12 weeks and 12 weeks of cortical bone and cancellous bone data worse than the control 12-week, 2D and 3D results of a similar trend in the results. Disuse and shows a lack of estrogen lead to the rapid loss of bone mass and bone tissue function of the degradation of mechanical properties.In short, the appeals conclusions can be drawn. For mechanical loading of osteoporosis caused by a lack of estrogen and disuse, and a very good therapeutic effect to a large extent, reduce the loss of bone mass and improving bone structure, increasing the mechanical properties of bone. There is also a relatively important in the clinical significance.